Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Factors that Influence the Activity of A Candida Lipase

Candida속(屬) Lipase의 활성(活性)에 영향(影響)을 미치는 제인자(諸因子)에 관한 연구(硏究)

  • Kim, S.Y. (College of Agriculture, Choongnam University) ;
  • Park, Y.J. (College of Agriculture, Choongnam University) ;
  • Lee, C.Y. (College of Agriculture, Seoul National University)
  • Published : 1971.12.30
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Abstract

An enzyme preparation from a newly isolated Candida that showed a high lipase activity was subjected to examination of its reaction rate under various conditions. The original and a diluted enzyme solutions showed the zero order curve starting at the point of 50 minutes in time. When PVA was used as an emulsifyer more activity was observed than the case of gum arabic. The optimal temperature and pH were $37{\sim}40^{\circ}C$ and 7.0, respectively. Oleic acid as a fatty acid conferred on the enzyme an inhibitory action while calcium ion a positive one. Sodium cholate yielded an increase in reaction rate at the first stage.

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