Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Studies on the Anthocyanins in Wild Vines (Vitis amurensis Ruprecht) - (Part 2) Identification of Anthocyanins in Wild Vines -

머루(Vitis amurensis Ruprecht) Anthocyanin에 관(關)한 연구(硏究) - [제 2 보(第 2 報)] 머루 Anthocyanin의 구조(構造) 확인(確認) -

  • Hwang, In-Kyeong (Dept. of Food and Nutrition, College of Home Economics, Seoul National University) ;
  • Ahn, Seung-Yo (Dept. of Food and Nutrition, College of Home Economics, Seoul National University)
  • 황인경 (서울대학교 가정대학 식품영양학과) ;
  • 안승요 (서울대학교 가정대학 식품영양학과)
  • Published : 1975.12.30
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Abstract

The studies on the structure of anthocyanins in wild vines have beer carried out. The pigments were extracted with 0.5% HCl in methanol from the wild vines which were wildly grown in the mountain area of Korea and purified with Amberlite IRC-50 canon exchange resin. The individual pigments were separated by paper chromatography. Sever. pigments were identified by partial acid hydrolysis, sugar moiety, alkaline degradation, $R_f$ values of paper chromatography in various solvents and absorption spectra in the visible region. The identified pigments were delphinidin 3-monoglucoside, petunidin 3-monoglucoside, cyanidin 3-monoglucoside, delphinidin 3, 5-diglucoside, malvidin 3-monoglucoside, petunidin 3, 5 diglucoside and malvidin 3, 5-diglucoside.

우리나라의 산간지방(山間地方)에 야생(野生)하는 머루중(中)의 anthocyanin의 구조(構造)를 확인(確認)하였다. 머루에서 0.5% HCl methanol로 추출(抽出)하여 얻은 색소(色素) 추출액(抽出液)을 Amberlite IRC-50 이온 교환수지관(交換樹脂管)을 통과(通過)시켜 정제(精製)하고, Paper chromatography에 의하여 개별색소(個別色素)를 분리(分離)하였다. 각(各) 개별색소(個別色素)에 대하여 부분(部分) 산가수분해(酸加水分解), 결합당(結合糖)의 확인시험(確認試驗), aglycone의 alkali 분해(分解), paper chromatography에서 얻어진 $R_f$치(値), 가시부(可視部)에서의 흡수(吸收) spectra 측정(測定) 등에 의하여 구조(構造)를 확인(確認)하였다. 확인(確認)된 머루중(中)의 7가지 색소(色素)는 delphinidin 3-monoglucoside, petunidin 3-monoglucoside, cyanidin 3-monoglucoside, delphinidin 3,5-diglucoside, malvidin 3-monoglucoside, petunidin 3,5-diglucoside 및 malvidin 3,5-diglucoside 이었으며 cyanidin 3,5-diglucoside로 추측되는 색소(色素) 4-b는 확인(確認)되지 못하였다.

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