Isolation, purification and characterization of phytohemagglutinating proteins from Korean natural products

초록

Seeds or beans of 55 plants belonging to 31 families were screened by using several different types of red blood cells to find new lectins. In this paper, white kidney been (phaseolus vulgaris C.) was chosen to study biochemical properties of hemagglutinating proteins(lectins). An anion exchanger, DEAE Sephadex A-50, and polyacrylamide disc gel electrophoresis were main techniques used. From three main fractions eluted by stepwise NaCl gradient in 25mM Tris-HCI buffer on DEAE Sephadex column, principal lectin was identified.

키워드

• Haemagglutionation proteins-Particular compounds such as enzymes;
• antibodies and lections-Phytohemagglutinin-Erythroagglutinin-Erythroagglu-tinating activity-Serial two-fold dilutions method on a microtiter system -White kidney beans-$(NH_4)_2SO_4$ precipitate-DEAE Sephadex A-50 colinm-$A_{280}$-Stepwise increasing NaCl gradient-Polyacrylamide disc gel electrophoresis;