Abstract

To study effect of dietary polyunsaturated fatty acid and $\omega$-tocopherol content on lipid peroxidation in rat liver, rats were fed for 3, 6 and 9 weeks with normal tocopherol diet added 40mg of DL-$\alpha$-tocopherol/kg of diet (PF group), high tocopherol eit 200mg of DL-$\alpha$-tocopherol/kg of diet(PFE group), low tocopherol diet without addition of DL-$\alpha$-tocopherol to diet(PFO group), and control diet added 40mg of DL-$\alpha$-tocopherol/kg of diet(control group). Each diet group supplied 45% of total calorie from corn oil except control group which supplied 12% of total calorie from corn oil. After each feeding period, lipid peroxide and tocopherol contents were measured in the liver as well as activities of glutathione peroxidase and superoxide dismutase. PF group had almost the same contents of liver peroxide, tocopherol contents and activities of glutathione peroxidease and superoxide dimutase as control group, while PFE group had higher tocopherol content and glutathione peroxidase activity and lower lipid peroxide contents and superoxide dismutase activity than control group. On the other hand, changes in the values of PFO group were opposite to those of PFE group. Differences in the values among groups were more pronounced as feeding period became longer.

High PUFA식이와 vitamin E 함량이 흰쥐 간장내의 지질과산화에 미치는 영향을 알아보기 위하여, 45Kcal% 고불포화 옥수수유의 정상적 vitamin E함량을 가한 식이군(PF군)과 45Kcal% 고불포화 옥수수유의 가한 PF군에 vitamin E를 5배 첨가한 식이군(PFE군), 45Kcal% 고불포화 옥수수유 식이에 vitamin E를 가하지 않은 식이군 (PFO군)을 실험군으로 하여 3주, 6주 및 9주간 사육한 후 간장에서의 vitamin E 함량과 과산화지질량 및 SOD와 GPX활성을 조사하였다. High PUFA식이에 vitamin E를 정상적으로 가한 PF군은 대조군과 차이가 나타나지 않았으나, vitamin E를 충분히 첨가한 식이 PFE군은 식이내 vitamin E 공급이 부족한 PFO군보다 vitamin E 함량과 GPX활성이 증가하였고, SOD활성과 과산화지질향은 현저하게 감소하였다. 식이 투여기간별로 PF군과 PFE군에 비해 PFO군이 실험기간이 경과할수록 vitamin E 함량과 GPX활성이 감소하였으나, SOD활성과 과산화지질향은 현저히 증가하였다.

Keywords