초록
인삼의 주요 polyacetylens 화합물인 panaxynol, panaxydol 및 panaxytriol의 정량을 위한 GC 분석조건을 확립하였다. 이 때, GC column과 검출기는 SPB-1 fused silica capillary(0.25 mm i, d. $\times$30m, Supelco)와 불꽃이온화검출기(FID)를 사용하였으며 column oven의 온도는 $250^{\circ}C$ 등온이었다. Panaxynol, panaxydol 및 panaxytriol peaks의 retention time은 TMS 유도체화 전의 경우에는 각각 4.2, 5.1 및 6.9분이었으며 TMS유도체화 후에는 각각 4.5, 5.4 및 7.4 분이었다. TMS 유도체화 전의 panaxynol, panaxydol 및 panaxytriol의 정량가능 최소농도는 각각 20, 50 및 100ppm 이었던 데에 비하여, TMS 유도체화 후에는 세 화합물의 정량가능 최소농도가 모두 5ppm의 낮은 수준을 나타내었다. 한편, 홍삼 중의 panaxynol, panaxydol 및 panaxytriol 함량 분석값은 각각 724, 721 과 71$\mu\textrm{g}$/g이었다.
A gas chromatographic method was developed for determination of the concentration of three major polyacetylene compounds in ginseng roots---panaxynol, panaxydol and panaxytriol. The column and the detector used were an SPB-1 fused silica capillary (0.2mm i.d.$\times$30m, Supelco) and a flame ionization detector (FID). The column oven temperature was kept at $250^{\circ}C$ isothermally The retention times of panaxynol, panaxydol and panaxytriol peaks were 4.2,5.1 and 6.9 min before TMS-derivatitration and 4.5,5.4 and 7.4 min after TMS-derivatization, respectively. The minimum determinable concentrations of panaxynol, panaxydol and panaxytriol before TMS-derivatiEation were at the 20, 50 and 100 ppm levels, while the concentrations of panaxydol and panaxytriol as well as panaxynol after TMS- derivatisation could be towered to the 5 ppm level. The panaxynol, panaxydol and panaxytriol contents in red ginseng were determined by use of this method after TMS-derivatization : the amounts obtained were 724, 721 and 71$\mu\textrm{g}$/g, respectively.