Journal of Biomedical Engineering Research (대한의용생체공학회:의공학회지)

The Korean Society of Medical and Biological Engineering (대한의용생체공학회)
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Endothelial Cell Seeding onto Extracellular Matrix for Development of Polyurethane Vascular Prosthesis

폴리우레탄 인공혈관을 위한 extracellular matrix 기질상의 내피세포이식

  • Lee, Yoon-Shin (Institute and Department of Biomedical Engineering College of Medicine Seoul National University) ;
  • Park, Dong-Kook (Institute and Department of Biomedical Engineering College of Medicine Seoul National University) ;
  • Min, Byoung-Goo (Institute and Department of Biomedical Engineering College of Medicine Seoul National University)
  • 이윤신 (서울대학교 의과대학 의공학연구소) ;
  • 박동국 (서울대학교 의과대학 의공학연구소) ;
  • 민병구 (서울대학교 의과대학 의공학연구소)
  • Published : 1991.09.01
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Abstract

Many experiments about endothelial cell seeding on artificial vessels were studied and conducted For this one or a combination of the extramatrix was used for the underlying matrix. But we used the whole ECM(extracellular matrix) that made excreated from flbroblasl. In thls study, we obtained human adult omental microvascular endothelium by collagenase digestion and used polyurthane sheets in order to make a new artificial vessel material. We cultured fibroblast on the polyurethane and gelatin - coated polyurethane. After confluent ingrowth we treated the polyure thane with triton in order to destroy the cytoskeleton and nucleus. We observed the preformed extra cellular matrix on the ployurethane and cultured the isolated microvascular endothelium. We also ok served the growth of microvascular endothelium on the polyurethane and gelatin. We conclude that the use of the whole ECM is promising fair as a new underying substrate for endothelial cell seeding on artificial vessels.

Keywords

References

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