Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Identification of the Actinomycetes Strain No. 497, Isolated from Soil, Producing Actinomycin Antibiotic MT-497

Actinomycin계열 항생물질 MT-497 을 생산하는 방선균 분리주 No.497의 동정

  • 안종석 (KIST 유전공학연구소 미생물공학연구실) ;
  • 이영선 (KIST 유전공학연구소 미생물공학연구실) ;
  • 안순철 (KIST 유전공학연구소 미생물공학연구실) ;
  • 이정형 (KIST 유전공학연구소 미생물공학연구실) ;
  • 이지행 (KIST 유전공학연구소 미생물공학연구실) ;
  • 윤병대 (KIST 유전공학연구소 미생물공학연구실) ;
  • 민태익 (KIST 유전공학연구소 미생물공학연구실)
  • Published : 1991.12.01
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Abstract

Identification of the Actinomycetes isolate strain No. 497 producing an actinomycin antibiotic MT-497 was performed by ISP and chemotaxonomic methods. The strain Nu. 497 formed various shapes of sclerotia and smooth surface spore. Menaquinone MK-9 ($H_6, H_8$) and iso-, anteiso-branched $C_{15}C_{17}$ fatty acids were detected from whole cell extract. The wall chemotype of stram No. 497 was decided as wall chemotype I from the analysis of DAP isomer, peptidoglycan type and sugar pattern. From these morphological, chemotaxonomic characteristics and analysis of various physiological characteristics. the strain No. 497 was identified as Streptomyces nigrifaciens.

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