Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation of Neutral Protease Hyperproducing Bacillus sp. KN103N and Some Properties of the Enzyme

中性 Protease 高 生産性 Bacillus sp. KN103N의 分離 및 酵素의 特性

  • Kim, Hong-Rip (Fermentation Technology Laboratory, R & D Center, Pacific Chemical Co.Ltd.) ;
  • O, Pyong-Su (Fermentation Technology Laboratory, R & D Center, Pacific Chemical Co.Ltd.)
  • 김홍립 (태평양화학(주) 기술연구소 발효광학연구실) ;
  • 오평수 (태평양화학(주) 기술연구소 발효광학연구실)
  • Published : 1991.04.01
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Abstract

A bacterial strain KN, which highly produced a protease, was isolated from several soil samples and identified to to belong to the genus Bacillus. We selected mutant strain Bacillus sp. KN103N, which was hyperproducer of protease and was resistant to D-cyclowerine, from the strain KN by several steps of mutagenesis. Neutral protease productivity of mutant strain KN103N was about 55 times as much as that of the original strain KN. The optimum pH and temperature for the enzyme activity were 7.0 and 50$^{\circ}C$, respectively and the enzyme was relatively stable at pH6.0~8.0 and below 40$^{\circ}C$. The enzyme was inactivated by EDTA, but not by DFP. These results indicate that the enzyme from Bacillus sp. KN103N was a neutral (metallo-) protease.

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