Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation of Thermostable ${\alpha}$-Amylase Hyperproducing Bacillus sp. No. 32H417 and Some Properties of the Enzyme

耐熱性 ${\alpha}$-Amylase 高 生産性 Bacillus sp. No. 32H417의 分離 및 酵素 特性

  • Kim, Moo-Sung (Fermentation Technology Laboratory, R & D Center, Pacific Chemical Co. Ltd.) ;
  • O, Pyong-Su (Fermentation Technology Laboratory, R & D Center, Pacific Chemical Co. Ltd.)
  • 김무성 (태평양화학(주) 기술연구소 발효공학연구실) ;
  • 오평수 (태평양화학(주) 기술연구소 발효공학연구실)
  • Published : 1991.04.01
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Abstract

A bacterial strain NO. 32 which produced thermostable ${\alpha}$-amylase was isolated from soil and identified to genus of Bacillus. To enhance ${\alpha}$-amylase productivity, a successive mutation of Bacillus sp. No. 32 was attempted with treatment of N-methyl-N'-nitro-N-nitrosoguanidine (NTG). The resulting mutant, Bacillus sp. No. 32H417, which is risistant to refampicin and deficient in spore formation, produced about 90-fold high level of ${\alpha}$-amylase when compared with parental strain. The properties of the enzyme for thermostability were investigated. The optimal temperature and pH for enzyme reaction were 95$^{\circ}C$ and pH6.5, respectively, in the presence of 0.3mM $Ca^{2+}$ as an effective stabilizer.

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