Abstract

To investigate the growth effect of branched oligosaccharides on the principal intestinal microorganisms, Bifdobacterium, Lactobacillus, Clostridium, Escherichia, Eubactemum, Enterucoccus, Staphylococcus and Bacteroides were cultivated on a medium containing branched oligosaccharides and panose. B. adolescentis, B. logum and L. aciduphilus grew effectively on the medium containing panose, while C. pe@igens, C. paraputrificum, Bac. fragilis and S. aurezds did not. The content of panose decreased greatiy in the culture broth of branched oligosaccharides of B, adolescentis, but it remained in the culture of C. perfringens. The results indicated that panose was consumed effectively by B. adolescentis, but not utilized by C. perfringens. 3. adolescentis still grew on the panose remained in the broth of mixed cultivation of B. adolescentis and C. perfn'ngens. Therefore, panose and branched oligosaccharides seem to promote selectively the growth of B. adolescentis in the human intestine.

Panose와 분지 올리고당 혼합당을 탄소원으로한 PYF당 검색용 배지에서 장내 주요 세균인 Bifidobacterium, Lactobacillus, Clostridium, Escherichia, Eubacterium, Enterococcus, Staphylococcus, Bacteroides의 생육을 조사하였다. B.adolescentis, B.bifidum, L.acidophilus 등은 panose 배지에서 lag phase가 긴편이었으나 생육이 양호함을 알았고 C.perfringens, C.paraputrificum, Bac.fragilis, S.aureus 등은 자라지 못했다.

Keywords

• Panose;
• branched oligosaccharides;
• Bifidobacterium adolescentis