Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Purification and Chemical Identification of the Inhibitor on Bleb Formation of K562 Cell Induced by Phorbol Ester from Actinornycetes Isolate No. 1882-5

방선균 분리주 No. 1882-5로부터 Phorbol Ester에 의해 유도되는 K562 Cell의 소포형성을 억제하는 물질의 분리와 동정

  • 안종석 (한국과학기술연구원 유전공학연구소 미생물공학실) ;
  • 안순철 (한국과학기술연구원 유전공학연구소 미생물공학실) ;
  • 박문수 (한국과학기술연구원 유전공학연구소 미생물공학실) ;
  • 김보연 (한국과학기술연구원 유전공학연구소 미생물공학실) ;
  • 민태익 (한국과학기술연구원 유전공학연구소 미생물공학실) ;
  • 이현선 (한국과학기술연구원 유전공학연구소 미생물공학실) ;
  • 오원근 (한국과학기술연구원 유전공학연구소 미생물공학실)
  • Published : 1992.10.01
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Abstract

We isolated Actinomycetes strain No. 1882-5, which produces the inhibitor on the bleb formation of K562 cell induced by phorbol ester, from soil sample. Through solvent extraction, Amberlite XAD-4, silica and Lobar low pressure LC, antifungal antibiotic MT 1882-1 and bleb forming inhibitor MT 1882-II were purified from strain No. 1882-5. MT 1882-1 was identified as piericidin $A_{1}$($C_{25}H_{37}0_4N$, M.W. 415) and MT 1882-11 as glucopiericidin A($C_{31}H_{47}0_9N$, M.W. 577) from the analysis of physico-chemical properties and UV, $^1H-NMR$, $^13C-NMR$, and mass spectra of these compounds.

Phorbol ester에 의해 유도되는 K562 cell의 소포 형성을 저해하는 물질을 생산하는 방선균 분리주 No.1882-5를 토양으로부터 분리하였다. 분리주 No. 1882-5로부터 용매추출, Amberlite XAD-4, silica, Lobar low pressure LC를 거쳐 항진균 활성물질 MT-1882-I과 K562 cell의 소포형성을 억제하는 활성물질 MT 1882-II를 분리하였다. 이 물질들에 대한 이화학적 성질의 조사와 UV, $^1H-NMR$, $^13C-NMR$, mass spectrum의 분석에 의하여 MT 1882-II은 piericidin $A_{1}$($C_{25}H_{37}0_4N$, MW 415), MT 1882-II는 glucopiericidin A($C_{31}H_{47}0_9N$, MW 577)로 동정하였다.

Keywords

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