Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Elucidation of the translocation of phosphamidon used for the control of pine leaf gall midges (Thecodiplosis japonensis Uchida et Inouye) in the trunks of pine trees by means of a radiotracer. Part 1. Translocation as affected by pine tree species and the treated season

방사성 추적자에 의한 솔잎혹파리 방제용 살충제 phosphamidon의 소나무 수간이동 구명 : I. 소나무 품종 및 계절별 이행

  • Lee, Jae-Koo (Departments of Agricultural Chemistry, Chung Buk National University) ;
  • Lee, Hyung-Rae (Departments of Agricultural Biology, Chung Buk National University) ;
  • Kyung, Kee-Sung (Departments of Agricultural Chemistry, Chung Buk National University) ;
  • Byun, Byung-Ho (Forest Research Institute, Forestry Administration)
  • 이재구 (충북대학교 농과대학 농화학과) ;
  • 이형래 (충북대학교 농과대학 농생물학과) ;
  • 경기성 (충북대학교 농과대학 농화학과) ;
  • 변병호 (산림청 임업연구원)
  • Published : 1993.12.31
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Abstract

In order to elucidate the translocation of the systemic insecticide, phosphamidon (2-chloro-2-diethylcarbamoyl-1-methylvinyl dimethyl phosphate), treated to pine trees against pine leaf gall midges (Thecodiplosis japonensis Uchida et Inouye), $[vinyl,\;carbonyl-^{14}C]$ phosphamidon was implanted into the trunks of 10-year-old Korean red pine (Pinus densiflora Sieb. et Zucc.) and Japanese black pine (Pinus thunbergii Parl.), respectively. The upward movement rates of the chemical within Korean red pine in July and Japanese black pine in December were ca. 10 cm/hr and 2 cm/hr, respectively. The original radioactivity level persisted up to 75 days after treatment throughout the whole tree in Korean red pine in July, whereas it did up to 150 days in Japanese black pine in December. The chemical was translocated up to the top at the insecticidal level within 3 days after treatment in July, whereas in December it was within 15 days. The translocation of the chemical was much dependent upon pine tree species and the treated season. Methanol was suitable for the extraction of phosphamidon and its metabolites from pine needles. Autoradiography of the methanol extracts of pine needles collected from the treated pine trees proved that phosphamidon broke down very quickly within pine trees (ca. 80% metabolized within 7 days).

솔잎혹파리(Thecodiplosis japonensis Uchida et Inouye) 방제용으로 소나무의 수간에 주입된 침투성 살충제 phosphamidon(2-chloro-2-diethylcarbamoyl-1-methylvinyl dimethyl phosphate)의 이행 및 분포를 구명하기 위하여 $[vinyl,\;carbonyl-^{14}C]phosphamidon$을 약 10년생의 적송과 해송에 각각 수간주사하였다. 이 약제의 최고이행속도는 적송(Pinus densiflora Sieb. et Zucc.)과 해송(Pinus thunbergii Parl.)에서 각각 약 10 cm/hr(7월)와 2 cm/hr(12월)이었다. 처리된 방사능은 하계의 적송에서는 75일까지 일정한 수준을 유지하였으나 동계의 해송에서는 150일까지 유지하였다. 본 약제가 살충가능한 농도로 top에 도달하는데 걸리는 기간은 적송(7월)에서는 3일 이내인 반면 해송(12월)에서는 15일 이내이었으며, 그 이행정도는 약제처리시기 및 수종에 의하여 영향을 받았다. 솔잎에 잔류하는 phosphamidon과 그 대사산물은 methanol로 잘 추출되었으며, 솔잎시료의 methanol 추출액을 autoradiography한 결과 phosphamidon은 소나무내에서 신속히 분해되어 7일 내에 약 80% 정도가 분해되었다.

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