Abstract

In order to elucidate the metabolism of the systemic insecticide, phosphamidon(2-chloro-2-diethylcarbamoyl-1-methylvinyl dimethyl phosphate), treated to pine trees against pine leaf gall midges (Thecodiplosis japonensis Uchida et Inouye), $[vinyl,\;carbonyl-^{14}C]$phosphamidon was implanted into the trunks of 10-year-old Korean red pine (Pinus densiflora Sieb. et Zucc.) and Japanese black pine (Pinus thunbergii Parl.), respectively. This chemical was degraded very quickly in pine trees after implanting, as confirmed by TLC/autoradiography of the extracts of pine needles. Phosphamidon metabolites in phosphate buffer extracts of pine needles include the major metabolite, ${\alpha}-chloroacetoacetic$ acid diethyl-amide, ${\alpha}-chloroacetoacetic$ acid ethylamide, 3-hydroxy-N,N-d iethylbutanamide, acetoacetamide, and trimethyl phosphate. The metabolism within pine trees is expected to be similar to this. Based on these findings, it is believed that the major pathway leading to the metabolites would be related to the P-O-vinyl hydrolysis of the chemical structure.

솔잎혹파리(Thecodiplosis japonensis Uchida et Inouye) 방제용으로 소나무의 수간에 주입된 침투성 살충제 phosphamidon(2-chloro-2-diethylcarbamoyl-1-methylvinyl dimethyl phosphate)의 대사를 구명하기 위하여 $[vinyl,\;carbonyl-^{14}C]phosphamidon$을 약 10년생 소나무의 수간에 주사하고 그 솔잎 추출액을 autoradiography한 결과 phosphamidon은 소나무 체내에서 신속히 분해됨을 알 수 있었다. 솔잎의 phosphate buffer 추출액 중에서 phosphamidon은 주대사산물인 ${\alpha}-chloroacetoacetic$ acid diethylamide를 비롯하여 ${\alpha}-chloroacetoacetic$ acid ethylamide, 3-hydroxy-N,N-diethylbutanamide, acetoacetamide, 그리고 trimethyl phosphate 등의 대사산물을 생성하였으며, 이 대사작용은 소나무 체내에서도 유사하리라 추정된다. 또한 주 대사경로는 구조식중 P-O-vinyl 결합의 가수분해와 관련있는 것으로 보인다.

Keywords

• Phosphamidon;
• metabolism;
• Korean red pine;
• Japanese black pine