Applied Biological Chemistry
- Volume 37 Issue 4
- /
- Pages.243-247
- /
- 1994
- /
- 2468-0834(pISSN)
- /
- 2468-0842(eISSN)
Separation and Purification of Endo-polygalacturonase from Korean Jujube
한국산 대추로부터 Endo-polygalacturonase 분리 및 정제
-
Choi, Cheong
(Department of Food Science & Technology, Yeungnam University)
;
- Chun, Sung-Sook (Department of Food Science & Technology, Yeungnam University) ;
- Cho, Young-Je (Department of Food Science & Technology, Yeungnam University) ;
- Woo, Heui-Seob (Department of Food Science & Technology, Yeungnam University) ;
-
Kim, Tae-Wan
(Department of Food Science & Technology, Yeungnam University)
;
- Heo, Young-Hoon (Department of Food Science & Technology, Yeungnam University)
-
최청
(영남대학교 식품가공학과)
;
- 천성숙 (영남대학교 식품가공학과) ;
- 조영제 (영남대학교 식품가공학과) ;
- 우희섭 (영남대학교 식품가공학과) ;
-
김태완
(영남대학교 식품가공학과)
;
- 허영훈 (영남대학교 식품가공학과)
- Published : 1994.08.31
Abstract
Endo-polygalacturonase was purified from Jujube. The purification procedures included DEAE-cellulose ion exchange chromatography and gel filtration on Sephdex G-100. Enzyme was purified as a single protein band and purification yield was about 6%. When the purified enzyme was applied to sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the molecular weight was estimated about 19,000. Purified enzyme formed hexagonal board type.
한국산 대추로부터 조직의 연화에 작용하는 endo-polygalacturonase를 gel filtration과 이온교환크로마토그라피에 의하여 단일 단백질로 정제할 수 있었고, 수율은 약 6% 정도였다. 정제된 이 효소는 sodium dodecyl sulfate-polyacrylamide gel electrophoresis에 의하여 분자량이 약 19,000정도의 monomer로 확인되었고, 결정구조는 육각판상 모양을 나타내었다.