Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Bacteria-Induced Antibiotic Peptide, Protaecin from the White-Spotted Flower Chafer, Protaetia brevitarsis

Protaetia brevitarsis가 생산하는 세균 유도성 항생황성물질, Protaecin

  • Park, Ho-Yong ;
  • Park, Doo-Sang ;
  • Park, Soon-Sik ;
  • Oh, Hyun-Woo ;
  • Shin, Sang-Woon ;
  • Lee, Hyeong-Kyu ;
  • Joo, Chang-Kyeong ;
  • Hong, Soon-Duck
  • 박호용 (KIST 유전공학연구소) ;
  • 박두상 (경북대학교 미생물학과) ;
  • 박순식 (KIST 유전공학연구소) ;
  • 오현우 (KIST 유전공학연구소) ;
  • 신상운 (KIST 유전공학연구소) ;
  • 이형규 (KIST 유전공학연구소) ;
  • 주창경 (경북대학교 미생물학과) ;
  • 홍순덕 ((주)인터페이스 엔지니어링)
  • Published : 1994.02.01
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Abstract

The induced antibiotic peptides were isolatde from the white-spotted folwer chafer, Protaetia brevitarsis by injection of E. coli suspension to the larvae of the insect. The antibacterial activity of the peptides were assayed by the plate growth ingibition method, and were purified by ion-exchange chromatography, reversed-phase HPLC, ion-exchange HPLC and SDS-PAGE etc. The peptides were estimated as 9 kDa in molecular weight and named Protaecin I and Protaecin II, respectively. Protaecin I and II have strong antibacterial activities against Gram-positivie and/or Gram-negative bacteria, and they are stable in the heat treatment and in the range of pH 2-12.

Keywords

References

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