Effect of pH on the Production and Characteristics of Protease by Bacillus sp. SH-8 and Bacillus sp. SH-8M

Bacillus sp. SH-8과 Bacillus SP. SH-8M의 Protease 생산 및 특성에 미치는 pH의 영향

  • 심창환 (경민전문대학 식품영양과) ;
  • 정광선 (강원대학교 발효공학과) ;
  • 신원철 (강원대학교 발효공학과) ;
  • 유주현 (연세대학교 식품공학과)
  • Published : 1994.02.01

Abstract

The production and the characteristics of protease produced by Bacillus sp. SH-8 and Bacillus sp. SH-8M were investigated under the different pH conditions. Bacillus sp. SH-8 and Bacillus sp. SH-8M showed the maximum activity of protease at 60 hours(70 units/ml) AND 96 houre(50 units/ml) cultivation, respectively, under the alkaline condition(pH 10.2). However, Bacillus sp. SH-8M exhibited the maximum activities in 8 days cultivation at pH 6.9 and in 6 days cultivation at pH 7.7 Bacillus sp. SH-8M showed the protease activity at the pH change from alkaline to neutral condition, whereas Bacillus sp. SH-8 did not. In addition. all the enzymatic characteristics of protease produced by Bacillus sp. SH-8 and Bacillus sp. SH-8M were similar with the regardless of different pH conditions.

Keywords

References

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