Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Sequence Analysis of $\beta$-Xylosidase Gene from Bacillus stearothermophilus

Bacillus stearothermophilus $\beta$-Xylosidase 유전자의 염기 서열 결정 및 분석

  • 오현주 (고려대학교 자연자원대학 유전공학과) ;
  • 최용진 (고려대학교 자연자원대학 유전공학과)
  • Published : 1994.04.01
Download PDF
⟨ Previous Next ⟩

Abstract

The neucleotide sequences of the xylA gene encoding $\beta $-xylosidase of Bacillus stearothermophilus and is its flanking regions were datermined. Three open reading frame(ORFs) were found, one of which(ORF1) appeared to code for the $\beta $-xylosidase. The 1830 base pair ORF1 encoded 609 amino acids starting from a TTG initiation codon. The molecular weight deduced from the nucleotide sequence(68 KD) was in agreement with that estimated by SDS-polyacrylamide gel electrophoresis of the purified enzyme(66 KD). The Shine-Dalgarno sequence(5'-AGGAGG-3') was found 11 bp upstream of the initiation codon. Further 15 bp upstream, there observed a potential transcription initiation signals. The putative -10 sequence(CATAAT) and -35 sequence(TTGTTA) coresponded closely to the consensus sequences for Bacillus subtilis RNA polymerase with major sigma factor. The guanine-plus-cytosine content of the coding region of the xylA gene was 56mol% while that of the third position of the codons was 63 mol%. Based on the comparison with the amino acid sequences of several other carbohydrate degrading enzymes, two conserved regions, possibly participating in the catalytic mechamism of $\beta $-xylosidase xylA, were identified in 278-298 and 329-350 regions of the translated xylA gene. The nucleotide sequence of the xylA was found to exhibit no homology to any other genes so far reproted.

Keywords

References

  1. Biosynthesis and biodegradation of wood commponents Biodegradation of hemicelluloses Dekker,R.F.H.;T.Higuchi
  2. J. Chem. Soc. v.12 Constitution of Xylan from Esparto Grass Chanda,S.K.;E.L.Hirst;J.K.N.Jones;E.G.V.Percival
  3. Microbial. Rev. v.52 Multiplicity of β-1.4-xylanase in microorganisms: functions and applications Ken K.Y. Wong;Larry,U.L.;John N. Saddler
  4. Kor. J. Appl. Microbial. Bioeng. v.20 Molecular cloning and expression of Bacillus stearothermophilus β-D-xylosidase gene in E. coli Oh,S.W.;S.S.Park;Y.I.Park;Y.J.Choi
  5. Kor. J. Appl. Microbiol. Biotechnol. v.20 Purification and characterization of exo-xylanase from Escherichia coli cells harboring the recombinant plasmid pMG1 Mun,A.R.;Y.J.Choi
  6. Meth. Enzymol. v.68 Plasmids of Escherichia coli as cloning vectors Bolivar,F.;K.Backman
  7. Gene v.19 The pUC plasmids, and M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers Vieira,J.;J.Messing
  8. Meths Enzymol. v.153 Production of single-stranded plasmid DNA Vieira,J.;J.Messing
  9. Molecular Clonig a laboratory manual Maniatis.T.;E.F.Fritsch;J.Sambrook
  10. Bacillus subtilis v.186 Necleotide sequences that signal the initiation of transcription and translation C.P.Moran Jr.;N.Lang;S.F.J.LeGrice;G.Lee;M.Stephens;A.L.Sonenshein;J.Pero;R.Losick
  11. J. Biol. Chem. v.256 Unique features in the ribosome binding site sequence of the gram-positive Staphylcoccus aureus β-latamase gene J.R.McLaughin;C.L.Murray;J.C.Rabinowitz
  12. Eur. J. Biochem. v.202 Sequence and properties of β-xylosidase from Bacillus pumilus IPO W.Z.XU;Y.Shima;S.Megoro;I.Urabe
  13. GENES IV B.Lewin
  14. J. Bacteriol. v.163 Nucleotide sequence and promoter region for the neutral protease gene from Bacillus stearothermophilus M.Takagi;T.Imanaka;S.Aiba
  15. Eur. J. Biochem. v.132 The amino acid sequence of the tyrosyl-tRNA synthetase from Bacillus stearothermophilus Winter,G.;G.L.E.Koch;B.Hartley;D.Barker
  16. J. Biol. Chem. v.259 Highguanine plus cytosine content in the third letter of codons of an extreme thermophile Kagaqu,Y.;H.Nojima;M.Ishizuka;T.Makajima;T.Yasuhara;T.Tanaka;T.Oishima
  17. J. Bacteriol. v.160 Cloning of the neutral protease gene of Bacillus subtilis and the use of the cloned gene to create an in vitro-derived deletion mutation Yang,M.Y.;E.Ferrari;D.J.Henner
  18. J. Bacteriol. v.158 Replacement of the Bacillus subtilis subtilisin structural gene with an in vitro-derived deletion mutation Stahl,M.L.;E.Rerrari
  19. J. Bacteriol. v.159 Genes for alkaline protease and neutral protease from Bacillus amyloliquefaciens contain an large open reading frame between the regions coding for signal sequence and mature protein Vasantha,M.;L.D.Thompson;C.Rhodes;C.Banner;J.Nagle;D.Filpula
  20. Gene v.18 Preferential codon usage in prokaryotic genes: the optimal codon-anticodon interaction energy and the selective usage in efficiently expressed genes Grosjean,H.;Fiers,W.
  21. Gene v.51 Homologous domains in Trichoderam reesei cellulolytic enzyme: gene sequence and expression of cellobiohydrolase Teeri,T.;P.Lehtovaara;S.Kauppinen;I.Salovouri;J.Knowles
  22. Science v.249 Three-dimensional structure of cellobiohydrolase II from Trichoderma reesei Rouvinen,J.;T.Bergfors;T.Teeri;J.K.Konwles;T.A.Jones
  23. Biochem. Biophys. Acta. v.870 Isolation and partial promary sequence of a xylanase from the yeast Cryptococcus albidus Morosoli,R.;C.Roy;M.Yaguchi
  24. Biochem. Biophys. Res. Commun. v.226 A fungal cellulase shows sequence homology with the active site of hen egg-white lysozyme Yaguchi,M.;C.Toy;C.F.Rollin;M.G.Paice;L.Jurasek
  25. EMBO J. v.3 Two different types of interveneing sequence in the glucoamylase gene from Aspergillus niger Boel,E.;M.T.Hansen;I.Hjort;N.P.Fiil
  26. Appl. Environ. Microbiol. v.57 Sequencing and expression of the Butyrivibrio fibrisolvens xyIB gene encoding a novel bifunctional protein with β-D-xylosidase and α-L-arabinofuranosidase activities Utt,E.A.;C.K.Eddy;K.F.Keshav;L.O.Ingram
  27. Mol. Gen. Genet. v.219 Cloning and sequencing of an endoglucanase(end1) gene from Butyrivibrio fibrisolvens H17c Berger,E.;W.A.Jones;D.T.Jones;D.R.Woods
  28. J. Bacteriol. v.172 Cloning, sequencing, and expression of a xylanase gene from the anaerobic rumen bacterium Butyrivibrio fibrisolvens Mannarelli,B.M.;S.Evans;D.Lee
  29. J. Bacteriol. v.162 Sequence of a cellulase gene of the thermophilic bacterium Clostridium thermocellum Benguin,P.;P.Cornet;J.P.Aubert
  30. J. Bacteriol. v.170 Purification of Clostridium thermocellum xylanase Z expressed in Esherichia coli and identification of the corresponding product in the culture medium of C. thermocellum Grepinet,O.;M.C.Chebrou;P.Beguin
  31. FEBS Lett. v.171 The complete nucleotide sequence of the xylanse gene(xylA) of Bacillus pumilus Fukusaki,E.;W.Panbangred;A.Shinmyo;H.Lkada
  32. Nucleic Acids. Res. v.14 Nucleotide sequence of the cellulase gene celD encoding endoglucanase D of Clostridium thermocellum Jolifff,G.;P.Benguin;J.P.Aubert
  33. Appl. Environ. Microbiol. v.56 Cloning, sequence analysis, and expression of genes encoding xylan-degrading enzymes from the thermophilus Caldocellum saccharolyticum Luthi,E.;D.R.Love;J.McAnulty;C.Wallace;P.A.Caughey;D.Saul;P.Bergquist
  34. Curr. Genet. v.12 Sequence and transcription of the β-glucosidase gene of Kluyveromyces fragilis cloned in Saccharomyes cerevisiae Raynal,A.;C.Gerbaud;M.C.Francingurd;M.Guerineau
  35. Nucleic Acids Res. v.12 Evidence for coding pattern on the non-coding strand of the Escherichia coli genome Allf-Steinberger,C.
  36. Kor. J. Appl. Microbiol. Bioeng. v.17 Molecular cloning and expression of a xylanase gene from thermophilic akaliphilic Bacillus sp. K-17 in E. coli Yu,J.;H.Chung;K.S.Chung;D.H.Oh
  37. J. Bacteriol. v.163 Nucleotide sequence of a Bacillus stearothermophilus α-amylase gene Nakajima,R.;T.Imanaka;S.Aiba