Extraction Yields of Hizikia fusiforme by Solvents and Their Antimicrobial Effects

추출 용매에 따른 톳 추출물의 수율 및 항균성 검정

  • KIM Soo-Hyun (Department of Food Science and Technology, Cheju National University) ;
  • LIM Sang-Bin (Department of Food Science and Technology, Cheju National University) ;
  • KO Young-Hwan (Department of Food Science and Technology, Cheju National University) ;
  • OH, Chang-Kyung (Department of Food Science and Technology, Cheju National University) ;
  • OH Myung-Cheol (Department of Food Science and Technology, Cheju National University) ;
  • PARK Che-Seok (Department of Food Science and Technology, Cheju National University)
  • Published : 1994.09.01

Abstract

Extraction yields of Hizikia fusiforme with four kinds of solvents and $0{\sim}90\%$ ethanol solutions, and antimicrobial activities of the extracts against Escherichia coli, Bacillus subtilis, Saccharomyces cerevisiae and Alternaria sp. were investigated. Determination of extraction yield with several solvents showed the highest value, $33\%$ (wt/wt), when distilled water was used. However, the extraction yields by hexane, ethyl ether and ethanol were less than $1\%$ (wt/wt). When dried Hizikia fusiforme was extracted at various concentrations of ethanol, 0 to $50\%$ solution gave similar yields and the extraction yields decreased rapidly as the ethanol concentrations increased above $70\%$, Ethanol-extract demonstrated antimicrobial activities against E. coli and B. subtilis, and ethyl ether- and hexane-extracts against E. coli. 70 and $90\%$ ethanol-extracts had potent antimicrobial activities against E. coli, and B. subtilis.

천연 보존료의 개발에 활용할 기초자료를 얻기 위하여, 제주도에서 많이 생산되고 있는 톳을 $40^{\circ}C$에서 용매별, 농도별로 추출하여 각각의 추출 수율을 측정하고, 이 추출물들의 항균성을 검색하였다. 용매별 건조톳의 추출 수율은 증류수인 경우 $33\%$로서 가장 높았고, 나머지 헥산, 에틸에테르, 에탄올인 경우 $1\%$ 이하로 매우 적었다. 에탄을 농도 별건조톳의 추출 수율은 $0\%$에서 $50\%$까지는 거의 비슷하였으나 $70\%$이상에서는 에탄을 농도가 증가할수록 급격히 감소하였다. 용매별 건조톳의 추출물들에 대하여 항균성을 검색한 결과 에탄올 추출물인 경우는 E. coli와 B. subtilis에 대하여, 에틸에테르와 헥산 추출물들인 경우는 E.. coli에 대하여 항균성을 보였다. 에탄올 농도 별 건조톳의 추출물들에 대한 항균성 검색 결과 70과 $90\%$ 추출물들이 E. coli와 B. subtilis에 대하여 뚜렷한 항균효과를 나타내었다.

Keywords