Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
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Comutagenic Effect of Sodium Chloride in the Salmonella/Mammalian Microsome Assay

Kim, So-Hee;Park, Kun-Young;Suh, Myung-Ja

  • Published : 1995.12.31

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Abstract

The mutagenic and comutagenic effects of sodium chloride (NaCl) were investigated in Salmonella/mammalian microsome assay system. The comutagenicity of NaCl on N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) along with the inhibiting ef-fect of vitamin C on its comutagenicity was also studied. Whether the activating enzyme mixture (S9 mixture) was absent or present in the system, NaCl revealed a slight mutagenic effect in the Salmonella typhimurium TA100, which exhibits a base-pair substitution mutagen. But the mutagenic effect of NaCl was not shown in the test strain of TA98 that detects a frameshift mutagen, suggesting that NaCl can be a weak base-pair substitution mutagen. NaCl also exhibited enhancing effect of the mutagenicities induced by aflatoxin $B_1(AFB_1)$ and MNNG. Its comutagenicity was remarkable especially on the direct mutagen of MNNG. As the concentrations of NaCl increased the comutagenic effect of NaCl for MNNG increased significantly (p<0.05). The revertant numbers were about 3 times when 15% of NaCl was added to the MNNG in the system compared to MNNG alone. However, vitamin C decreased the comutagenicity of the NaCl on the MNNG mediated mutagenicity.

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References

  1. Gann Monogr v.11 Epidemiology of stomach cancer Hirayama, T.
  2. Gann Monogr v.26 Cancer motality in Japan Kuroishi, T.;Tominaga, S.;Hirose, K.;Segi, M.
  3. Gann Monogr v.3 The epidemiology of cancer of the stomach in Japan: with special reference to the role of diet Hirayama, T.
  4. Bull. lnst. Public Health v.8 Studies of the causation of gastric cancer Sato, T.;Fukuyama, T.;Suzuki, T.;Takayanagi, J.;Murakami, T.;Shiotsuki, N.;Tanaka, R.;Tsuji, R.
  5. Fd. Chem. Toxicol. v.24 Diet and stomach cancer Miller, A.
  6. Gann v.74 Effect of high salt diet on rat gastric carcinogenesis induced by N-methy-N'-nitro-N-nitrosoguanidine Takahashi, M.;KoKubo, T.;Furukawa, F.;Kurokawa, Y.;Tatematsu, M.;Hayashi, Y.
  7. Gann v.75 Effects on sodium chloride, saccharin, phenobarbital and aspirin on gastric carcinogenesis in rats after initiation with N-methyN'-nitro-N-nitrosoguanidine Takahashi, M.;KoKubo, T.;Furukawa, F.;Hayashi, Y.
  8. Carcinogenesis v.12 Enhanced lipid peroxidation in rat gastric mucosa caused by NaCI Takahashi, M.;Hasegawa, T.;Furukawa, F.;Okamiya, H.;Shinoda, K.;lmaida, K,;Toyada, K.;Hayashi, Y.
  9. Salt and stomach cancer;Gastric carcinogenesis Joossens, J.V.;Kesteloot, H.;Reed, P.I.(ed.);Hill, M.J.(ed.)
  10. Mutat. Res. v.113 Revised methods for the Salmonella mutagenicity test Maron, D.M.;Ames, B.N.
  11. Factors modulating mutagenicity in microbial test;Short term test systems for detecting carcinogens Matsushima, T.;Sugimura, T.;Nagao, M.;Yahagi, T.;Shirai, A.;Sawamura, M.;North, K.H.(ed.);Garner, K.C.(ed.)
  12. Principles and procedures of statistics Steel, K.G.;Torrie, J.H.
  13. Nagoya Med. J. v.19 Periodical observations of the glandular stomach in rats treated with N-methy-N'-nitro-N-nitrosoguanidine Shirai, T.
  14. J. Natl. Cancer Inst. v.55 Effects in rats of sodium chloride on experimental gastric cancers induced by N-methyl-N'-nitro-N-nitrosoguanidine or 4-nitroquinoline 1-oxide Tatematsu, M.;Takahashi, M.;Fukushima, S.;Hananouchi, M.;Shirai, T.
  15. Enhancing effects of dietary salt on both initiation and promotion stages of rat gastric carcinogenesis;Dliet, nutrition and cancer Takahashi, M.;Hasegawa, K.;Hayashi. Y.(ed.);Nagao, M.(ed.);Sugimura, T.S.(ed.);Tomatis, J.(ed.);Wattenberg, L.W.(ed.);Wogan, G.N.(ed.)
  16. Oncology v.10 Vitamin C and cancer prevention Bright-See, E.
  17. Pure Appl. Chem. v.57 Carotenoids and cancer prevention experimental and epidemiological studies Mathews-Roth, M.M.
  18. Life Science v.27 Modulation of the effects of tumor therapeutic agents by vitamin C Prasad, K.N.
  19. The influence of nutritional status on pollutant toxicity and carcinogenicity;Nutrition and environmental health. vol.1 Calabrese, E.J.
  20. Nature v.268 Inhibition by L-ascorbate of bacterial mutagenesis induced by two N-nitroso compounds Guttenplan, J.B.