Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Optimum Production Condition of Oligosaccharide by Bacillus cereus IAM1072

Bacillus cereus IAM1072에 의한 올리고당 생산의 최적조건

  • 이명열 (충주산업대학교 식품공학과) ;
  • 정만재 (충북대학교 식품공학과) ;
  • 이신영 (강원대학교 발효공학과) ;
  • 강태수 (강원대학교 발효공학과) ;
  • 이웅수 (충주산업대학교 식품공학과) ;
  • 민윤식 (충주산업대학교 식품공학과)
  • Published : 1995.10.01
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Abstract

This study was carried out to produce the oligosaccharides(G2~G7) directly from the culture broth of Bacillus cereous IAM1072. The maximal production of oligosaccharides was obtained after cultivation at $30^{\circ}C$ for 24hrs in the mixture of 12% soluble starch, 1.5% peptone and 0.25% $K_2HPO_4$ adjusted to pH 7.0. Among the oligosaccharides produced $G_5$ oligosaccharide was purified. After methylation, the result of GC analysis was suggested to be oligosaccharide consisting of 5 glucose unit with ${\alpha}-1,4-glucosidic$ bond.

이 연구는 Bacillus cereus IAM1072의 배양액에서 직접 올리고당을 생산하기 위해 행하였다. 올리고당 생성 배양조건은 접종비 3%, pH, 7.0, 온도 $30^{\circ}C$, 배지액량 40ml, 배양시간 24시간이었으며, 올리고당 생성에 가장 효과적인 최적 배지조성은 12% soluble starch, 1.5% perptone, 0.25% $K_2HPO_4$이었다. 생성된 올리고당 중 $G_5$ 올리고당을 정제하였다. Methylation을 한 후 GC 분석을 한 결과 $G_5$ 올리고당은 glucose 단위가 5개로 구성된 ${\alpha}-1,4-glucosidic$ 결합한 올리고당이었다.

Keywords

References

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