The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Differentiation of Lentinus edodes Isolates in Korea by Isozyme Polymorphisms and Random Amplified Polymorphic DNA (RAPD) Analysis

Isozyme Polymorphism 및 Random Amplified Polymorphic DNA(RAPD) Pattern에 의한 표고 버섯 품종간 비교

  • Park, Won-Mok (Graduate School of Biotechnology, Korea University) ;
  • Ko, Han-Gyu (Department of Agricultural Biology, College of Natural Resource, Korea University) ;
  • Park, Ro-Jo (Forest Product Microbial Products Center, National Forestry Cooperatives Federation) ;
  • Hong, Ki-Sung (Forest Product Microbial Products Center, National Forestry Cooperatives Federation) ;
  • Kim, Gyu-Hyun (Department of Agricultural Biology, College of Natural Resource, Korea University)
  • 박원목 (고려대학교 생명공학원) ;
  • 고한규 (고려대학교 자연자원대학 농생물학과) ;
  • 박노조 (임업협동조합 임산미생물 사업소) ;
  • 홍기성 (임업협동조합 임산미생물 사업소) ;
  • 김규현 (고려대학교 자연자원대학 농생물학과)
  • Published : 1997.09.30
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Abstract

Sixty-three isolates of Lentinus edodes obtained from Korea were used to assess the genetic similarity by isozyme polymorphisms and random amplified polymorphic DNA patterns. The activities of esterase, peroxidase and acid phosphatase displayed 10, 7 and 3 distinct isozyme patterns, respectively. By combining the isozyme patterns obtained with the 3 enzymes, every isolate showed its own distinct electrophoretic phenotypes. A distance matrix calculated between all pairs of 63 electrophoretic phenotypes based on the presence or abscence of isozyme bands were analyzed by the group-average method. Results of the cluster analysis assinged the 63 phenotypes into six major groups. In the analysis of random amplified polymorphic DNA patterns, all isolates of Lentinus edodes were devided into five RAPD groups.

Isozyme PAGE에 의한 isozyme polymorphism 및 random amplified polymorphic DNA(RAPD) pattern의 비교를 통하여 한국에서 수집된 63개의 표고 버섯(Lentinus edodes)품종의 유연관계를 조사하여 보았다. NTSYS PC program을 통하여 비교에 사용되어진 3가지 isozyme 즉 esterase, peroxidase, acid phophatase 등은 각각 10, 7, 3개의 isozyme polymorphism type으로 나뉘어질 수 있었고, 이러한 3개의 isozyme type들은 group-average method를 이용하여 최종적으로 6개의 집단으로 구분되어질 수 있었다. 또한 RAPD를 통한 표고 품종간 유연관계 비교는 사용되어진 총 20개의 random primer 들중 3개의 primer(OPA 03, OPA 18, OPA 19)가 품종간 polymorphism을 보였고 사용되어진 전체 random primer의 PCR product들의 집단 분석을 통해 최종적으로 5개의 집단으로 구분할 수 있었다. 사용되어진 두 가지 방법 즉, isozyme polymorphism및 RAPD pattern에 의해 구분되어진 63개 표고 버섯 품종의 subgroup level에서의 절대적인 유사성은 관찰할 수 없었다. Yii-Mattila(1996) 등에 의하면 품종간 비교시 isozyme analyses에서와는 달리 RAPD analyses에서는 많은 수의 strain-specific band들이 얻어질 수 있고 이것이 두 방법간의 유사성을 관찰하지 못하게 하는 원인이라고 보고하였다.

Keywords

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