The Artificial Cultivation of Pleurotus eryngii (II) -Morphological Characteristics of Fruit Body and Cultural Conditions-

Pleurotus eryngii균의 인공재배 (II) -자실체의 형태적 특성 및 재배조건에 관하여-

  • Kim, Han-Kyoung (Division of Applied Microbiology, National Institute of Agricultural Science and Technology, R.D.A.) ;
  • Cheong, Jong-Chun (Division of Applied Microbiology, National Institute of Agricultural Science and Technology, R.D.A.) ;
  • Seok, Soon-Ja (Division of Applied Microbiology, National Institute of Agricultural Science and Technology, R.D.A.) ;
  • Kim, Gwang-Po (Division of Applied Microbiology, National Institute of Agricultural Science and Technology, R.D.A.) ;
  • Cha, Dong-Yeul (Division of Applied Microbiology, National Institute of Agricultural Science and Technology, R.D.A.) ;
  • Moon, Byung-Ju (Department of Agricultural Biology, College of Natural Resources and Life Science, Dong-A University)
  • 김한경 (농촌진흥청 농업과학기술원 응용미생물과) ;
  • 정종천 (농촌진흥청 농업과학기술원 응용미생물과) ;
  • 석순자 (농촌진흥청 농업과학기술원 응용미생물과) ;
  • 김광포 (농촌진흥청 농업과학기술원 응용미생물과) ;
  • 차동렬 (농촌진흥청 농업과학기술원 응용미생물과) ;
  • 문병주 (부산동아대학교 생명자원과학대학 농생물학과)
  • Published : 1997.12.30

Abstract

These studies were carried out to develop an artificial cultivation method. The diameter and thickness of pileus ranged $1.5{\sim}7.0\;cm$ and $0.8{\sim}3.0\;cm$, respectively. The diameters of stipe were $1.2{\sim}2.5\;cm$ and the lengthes were $4.5{\sim}9.0\;cm$. The spore fingerprint was white. The sizes were spore $10.8{\sim}12.2{\times}4.35{\sim}5.65\;{\mu}m$, basidia $50.0{\sim}59.2{\times}7.4{\sim}7.8\;{\mu}m$, nalsistidia $21.75{\sim}28.7{\times}4.8{\sim}6.1\;{\mu}m$, pileus hymenium cell $50.6{\sim}66.0{\times}4.4{\sim}6.7\;{\mu}m$, and stipe hymenium cell $28.6{\sim}33.0{\times}5.5{\sim}6.6\;{\mu}m$. The thirty percent mixture of rice and wheat bran into sawdust gives the high density of mycelia and the good development of fruiting structure. The optimum water contents of sawdust substrates were $60{\sim}65%$ in which condition the mycelium grows well and gives high density. In PP bottle cultivation, the first fruiting period was $6{\sim}8$ days earlier in nonscratching samples than scratching ones, but the quantity of fruiting body was higher in scratching samples than nonscratching ones. In the case of PP bag cultivation, the first fruiting was 10 days faster, and the quantity of fruiting bodies was 30% higher in samples with 30% wheat bran than those with rice bran. The fleshiness of stipe was $2{\sim}3$ times harder than that of pileus.

큰느타리버섯 균의 인공재배법 개발에 관한 연구를 수행한 결과 다음과 같이 몇가지 결과를 얻었기에 보고하고자 한다. 큰느타리버섯의 톱밥인공 재배한 자실체의 형태는 갓직경 $1.5{\sim}7.0\;cm$, 갓두께 $0.8{\sim}3.0\;cm$, 대직경 $1.2{\sim}2.5\;cm$, 대길이는 $4.5{\sim}9.0\;cm$였다. 포자의 문은 흰색, 포자의 크기는 $10.87{\sim}12.2{\times}4.35{\sim}5.65\;{\mu}m$, 담자기의 크기는 $50.0{\sim}59.2{\times}7.4{\sim}7.8\;{\mu}m$, 날시스티디아의 크기는 $21.75{\sim}28.7{\times}4.8{\sim}6.1\;{\mu}m$, 갓표피 상층세포의 크기는 $50.6{\sim}66.0{\times}4.4{\sim}6.7\;{\mu}m$, 대표피 세포의 크기는 $28.6{\sim}33.0{\times}5.5{\sim}6.6\;{\mu}m$이었다. 참나무톱밥에 미강과 밀기울을 30% 혼합했을 때 균사밀도와 자실체 발생이 좋았다. 톱밥배지의 최적수분 함량은 $60{\sim}65%$에서 균사생육이 빠르고 밀도도 양호하였다. PP병 재배시 균사배양 기간은 $20{\sim}22$일, 초발이 소요일수는 균긁기를 하지 않은 처리구에서 $6{\sim}8$일로 빨랐으나 자실체 수량은 균긁기한 처리구에서 높았다. PP봉지 재배는 참나무톱밥에 미강을 첨가한 구에서 초발이 소요일수가 10일로 빠르고, 자실체 수량은 밀기울 30% 처리구에서 높았다. 자실체 경도는 큰느타리에서 갓보다 줄기부분이 약 $2{\sim}3$배 정도 육질이 단단했다.

Keywords

References

  1. 한국균학회지 v.11 느타리버섯의 볏짚배지 발효방법에 관한 연구 정환채
  2. 느타리버섯 배지재료 개발 시험.농기연(생물부편) 정환채;박정식;박용환
  3. Flora Agaricina Neerlandica;Critical monographs on families of agarics and boleti occurring in the Netherlands Bas, C.;Kuyper, Th.W.;Noordeloos, M.E.;Vellinga, E.C.
  4. Fungi of Switzerland. vol.3 Boletes and Agarics Breitenbach, J.;Kranzlin, F.
  5. Mush sci v.IX Recherches experimentales sur les conditions D,ambiance requises pour la fructification du Pleurotus eryngii et de L,Agrocybe aegerita Cailleux, R.;Diop, A.
  6. Universita degli Studi di Perugia v.42 Chemical composition and nutritive value of several strains of Pleurotus eryngii, P. nebrodensis and P. ostreatus grown in a greenhouse. Armali delIa Facolta di Agraria Coli, R.;Granetti, B.;Damiani, P.;Fidanza, F.
  7. Cesk. Mykol. v.28 Pleurotus eryngii (DC. Ex. Fr.) Quel. in Slovakia Dermar, A.
  8. Biology and breeding of Pleurotus, in The Biology and Cultivation of Edible Mushroom Eger, G.; Chang, S.T.(ed.);Hayes, W.A.(ed.)
  9. J. Agri Lab (Japan) v.8 Physiological and Ecological studies on Lentinus edodes(Berk) Sing Ishikawa, H.
  10. Kor. J. Mycol. v.17 no.3 Studies on the Artificial Cultivation of Agrocybe aegerita(Brig) Sing using Pime Sawdust Substrate Kim, H.K.;Park, J.S.;Kim, Y.S.;Cha, D.Y.;Park, Y.H.
  11. J. Microbiol. Bioeng. v.9 no.3 Selection of Some for Artificial Cultivation from the Wild Mushroom Kim, S.S.;Kim, K.J.
  12. Agricultural Wastes v.18 no.1 Influence of gaseous phase, light and substrate pretreatment on frity-body formation lignin der-gra-dation and in vitro digestibility of wheat straw fermented with Pleurotus spp Kmara, D.;Zadrazil, F.
  13. Die Gattung Pleurotus Hilber, Oswald
  14. Standard Soil Color Charts Oyama, M.;Takahara, A.
  15. Studies on the Cultivation of Oyster Mushroom, Pleurotus ostreatus(Fr.) Quel. Using Rice as Growing Substrate. 1. Experiments on the Development of Growing Substrate. Res. Repo. O.R.D Park, Y.H.;Go, S.J.;Kim, D.S.
  16. Acta-Horticulturae v.361 The use of olive milling waste-water for the culture of mushroom on perlite Pompei, R.;Demontis, M.G.;Sanjust, E.;Rinaldi, A.;Ballero, M.;Serra, G.;Tognoni, F.;Leoni, S.
  17. CRC Critical in Food Science and Nutrition v.26 no.2 Pleurotus mushrooms. Part 1 A. Morphology, Life cycle, Taxonomy, Breeding, and Cultivation Rajarathnam, S.;Bano, Z.
  18. Canada Mycotaon v.25 Nematoctonus and the Tribe Resupinateae In Onterio Thorn, R.G.;Barron, G.L.
  19. Science and Cultivation of Edible Fungi Cultivation of Pleurotus species during winter in India Upadhyay, R,C.;Vijay, B.
  20. A briss der geographischen Verbreitung der Hutpilze in der Sowjetunion (Moskau -Leningrad) Vasikov, P.B.
  21. Bull. Nagano Veg. and Ornam. Crops Exp. Sta. Japan no.6 Cultivation Methods on Hypsizigus marmoreus. Kind and Characteristics of Nutritional Substances of Bottle Culture Media (2) Yoshimitsu, M.;Tamotsu, S.;Toshimi, Y.;Kimiyoshi, N.;Kobun, A.;Hideki, Y.;Yoichi, K.
  22. Mush Sci v.IX The Ecology and industrial production of Pleurotus ostreatus, Pleurotus florida, Pleurotus cornucopiae, and Pleurotus eryngii Zadrazil, F.
  23. Mushroom J. v.28 A cultivated Pleurotus eryngii for use in forest and uncultivable areas of temperate zone Zahooruddin Agha
  24. Science and Cultivation of Edible Fungi Pleurotus species of Greece: An evaluation of their morphological and Physiological characteristics Zervakis, G.;Balis, C.