Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Occurrence of Tn3 Sequence Upstream of aacC2 Gene in Gentamicin Resistance R Plasmids

Gentamicin 저항성 R 플라스미드에 존재하는 aacC2 유전자의 상류부위에서 Tn3의 출현

  • 한효심 (순천대학교 생물학과) ;
  • 김남덕 (순천대학교 생물학과) ;
  • 이영종 (순천대학교 생물학과, 광주보건전문대 임상병리학과) ;
  • 이효연 (순천대학교 자원식물학과) ;
  • 정재성 (순천대학교 생물학과)
  • Published : 1997.09.01
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Abstract

Two gentamicin resistance R plasmids, pGM5 and pGM6, containing aacC2 gene were selected from environmental isolates. The gentamicin resistance determinants of R plasmids were cloned into the BamHI site of pUC18. Restriction enzyme map of inserted region of recombinant plasmids, pSYS and pSY6, and PCR results indicated that Tn3 sequence was located upstream of gentamicin resistance gene. Based on the restriction maps and susceptibility tests, it was concluded that the sequence of bla and 3' inverted repeat of Tn3 play a important roles in the expression of gentamicin resistance gene.

병원하수에서 분리한 gentamicin 저항성 세균으로부터 aacC2 유전자를 가지고 있는 R 플라스미드 pGM5와 pGM6를 선발하였다. 이들 플라스미드에서 gentamicin 저항성 유전자를 포함하는 부분을 pUC18의 BamHI 자리에 클로닝하여 재조합 플라스미드 pSY5와 pSY6를 각각 얻었다. 재조합 플라스미드의 삽입된 부분에 대한 제한효소 지도를 통해 Tn3 염기서열이 aacC2 유전자의 상류부위에 위치하는 것을 알았다. 재조합 플라스미드의 gentamicin에 대한 민감성의 비교를 통해 Tn3의 bla 유전자 부분과 3'역행중복 부위의 염기서열이 gentamicin 저항성 유전자의 발현에 중요한 역할을 담당하고 있었다.

Keywords

References

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