Isolation and Identification of Bacterium Producing Extracellular Cytosine deaminase

세포외 Cytosine Deaminase 생산균의 분리 및 동정

  • 유대식 (계명대학교 자연과학대학 미생물학과) ;
  • 김대현 (동국전문대학 외식산업과)
  • Published : 1997.02.01

Abstract

A bacterium, strain YK 391 producing extracellular cytosine deaminase, has been isolated from soil sample collected near Taegu City and identified. The strain YK 391 was observed to be a motile Gram-negative rod, and did not produced capsule nor spore. The bacterium produced acid from glucose and trehalose, not from arabinose. Esculine was nto hydrolyzed. The isolate could grow anaerobically at 37$\circ $C, but not at 4$\circ $C. Palmitoleic and palmitic acids comprised over 80% of the fatty acid composition of the strain. The strain. The strain YK 391 was identified as Chromobacterium violaceum YK 391 based on its morphological and physiolohical characteristics, and on the fatty acid composition. The extracellular cytosine deaminase produced by Chromobacterium violaceum YK 391 is believed to be unique because it was active not only on cytosine and 5-fluorocytosine but also on cytidine.

Keywords

References

  1. Escherichia coli and Salmonella typhimurium v.1 Purines and pyrimidines Neuhard, J.;P. Nygaard;Neihardt F. C.(ed.)
  2. Protien, Nucleic acid, Enzyme v.14 Interrelationship of nucleotide metabolism in mammalian tissues Tatibana, M.
  3. Biochem. Pharmac. v.15 The metabolism of 5-fluorocytosine-2$^{14}C$ and of cytosine-$^{14}C$ in the rat and the disposition on 5-fluorocytosine-2$^{14}C$ in man Koechlin, B. A.;Rubio, F.;Palmer, S.;Gabriel, T.;R. Duschinsky
  4. Z. Biol. v.79 Uber das verhalten von pyrimidinderivaten in den organismen. I. Einfluss von hefe auf pyrimidinderivate Hahn, A.;W. Lentzel
  5. Z. Biol. v.83 Uber das verhalten von pyridinderivaten in den organismen. II. Einsirkung von Bacterium coli auf und cytosin Hahn, A.;L. Schafer
  6. Agr. Biol. Chem. v.39 Purification of cytosine deaminase from Serratia marcescens Sakai, T.;T. S. Yu;H. Tabe;S. Omata
  7. Agr. Biol. Chem. v.39 Purificatin of cytosine deaminase from Pseudomonas aureofaciens Sakai, T.;T. S. Yu;H. Tabe;S. Omata
  8. Agr. Biol. Chem. v.40 Kinetic properties of cytosine deaminase from Serratia marcescens Yu, T. S.;T. Sakai;S. Omata
  9. Agr. Biol. Chem. v.40 Kinetic properties of cytosine deaminase from Pseduomonas aureofaciens Yu, T. S.;T. Sakai;S. Omata
  10. Biochim. Biophys. Acta. v.719 Purification and some properties of cytosine deaminase from Salmonella typhimurium West, T.;M. S. Shanly;G. A. O'Donovan
  11. J. Amer. Chem. Soc. v.73 On the cytosine deaminase of yeast Kream, J.;E. Chargaff
  12. Biochem. v.10 Baker's yeast cytosine deaminase. Some enzymic properties and allosteric inhibition by nucleosides and nucleotides Ipata, P. L.;F. Marmochii;G. Magni;R. Felio;G. Polidoro
  13. J. Ferment. Bioeng. v.72 Purification and some properties of cytosine deaminase from Aspergillus fumigatus Yu, T. S.;J. K. Kim;T. Katsuragi;T. Sakai;K. Tonomura
  14. Kor. J. Microbiol. v.22 Isolation of extracellular cytosine deamlnase producing strain Arthrobacter sp. JH13 and cultural condition of it's enzyme production Jun, H. K.;J. H. Park
  15. Kor. J. Microbiol. v.23 Properties of extracellular cytosine deamlnase from Athrobacter sp. Jun, H. K.;J. H. Park;Y. Yeeh
  16. Kor. J. Microbiol. v.26 Optimum cultural conditions for production of extracellular cytosine deaminase by Bacillus polymyxa YL38-3 Yu, T. S.;T. H. Kim;J. M. Park;H. I. Song;K. T. Chung
  17. Kor. J. Microbiol. v.26 Enzymatic properties of extracellular cytosine deaminase Yu, T. S.;T. H. Kim;J. M. Park;H. I. Song;K. T. Chung
  18. Proc. 12th International. Congr. of Chemotherapy New antineoplastic chemotherapy without systemic side effects to the host. Current Chemotherapy and Immunotherapy Nishiyama, T.;Y. Kawamura;T. Ito;A. Ohyama;T. Katsaragi;T. Sakai
  19. Neurol. Med. Chirs. v.22 Antineoplastic effect of 5-fluorocytosine at cytosine deaminase on brain tumor Nishiyama, T.;Y. Kawamura;K. Kwamoto;H. Matsumura;N. Yamamoto;T. Ito;A. Ohyama;T. Katsaragi;T. Sakai
  20. Cancer Research v.45 Antineoplastic effects in rats of 5-fluorocytosine in combination with cytosine deaminase Nishiyama, T.;Y. Kawamura;K. Kwamoto;H. Matsamura;N. Yamamoto;T. Ito;A. Ohyama;T. Katsargi;T. Sakai
  21. Handbook of Chromatography Zweig, G.;J. Sherma
  22. Anal. Biochem. v.72 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing for the principle of protein-dye binding Bradford, B. M.
  23. J. Biol. Chem. v.246 Cytidine deaminase from Escherichia coli, Purification, properties, and inhibition by the potential transition state analog 3,4,5,6-tetrahydrouridine Cohen, R. M.;R. Wolfenden
  24. J. Clin. Microbiol. v.18 Single derivatization method for routine analysis of bacterial whole cell fatty acid methyl esters, including hydroxy acid Miller, L. T.
  25. 微生物の 分類と 同定(下) 長谷天 武治
  26. Bergey's Manual of Determinative Bacteriology (9th ed.) Holt, J. G.;N. R. Krieg;P. H. A. Sneath;J. T. Staley;S. T. Williams
  27. Agric. Biol. Chem. v.50 Cytosine demainase from E. coli-production, purification and some characteristics Katsuragi, T.;T. Sakai;K. Matsumoto;K. Totonomura