Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

김치에서 분리한 Lactobacillus sp. JC-7과 Lactobacillus acidophilus 88간의 Electrofusion 최적조건 설정

  • 조영배 (부산대학교 자연과학대학 미생물학과) ;
  • 최현정 (부산대학교 자연과학대학 미생물학과) ;
  • 백형석 (부산대학교 자연과학대학 미생물학과) ;
  • 전홍기 (부산대학교 자연과학대학 미생물학과)
  • Published : 1997.04.01
Download PDF
⟨ Previous Next ⟩

Abstract

A lactic acid bacterium was isolated from kimchi. The isolated strain was identified as the genus Lactobacillus through its morphological, cultural, and physiological characteristics and named as Lactobacillus sp. JC-7. The optimum conditions for the electrofusion between streptomycin (2.5 mg/ml) resistant mutant of Lactobacillus acidophilus 88 and kanamycin (600 $\mu$g/ml) resistant mutant of Lactobacillus sp. JC-7 were evaluated. The highest number of fusants were obtained at a capacitance value of 120 msec (1670 $\mu$F), a field strength of 100 V/cm, and a pulse controller setting of 72$\Omega$. The optimum pH of electroporation buffer was 7.5 and the concentration of divalent cation was 1 mM Mg$^{2+}. Electrofusants were efficiently obtained by addition 20% polyethylene glycol to elec- troporation buffer. The yield of fusion was better than that of using polyethylene glycol mediated chemical induction.

Keywords

References

  1. 식품과 영양 v.8 김치의 맛과 영양 이서래
  2. 식품 과학 v.21 김치 발효와 보존성 최신양
  3. 인하대학교 기초 과학 연구소 논문집 v.11 각 온도에서 김치 발효중 미생물의 천이 과정 박현근;임종락;한홍의
  4. 한국식품과학회 심포지움발표논문집(김치의 과학) 공장김치의 발효온도 및 포장방법별 성분과 미생물의 변화 신동화
  5. Agric. Biol. Chem. v.54 Transformation of Lactobacillus helveticus subsp. jugurti with plasmid pLHR by electroporation Hashiba, H. R.;Takiguchi;S. Ishii;K. Aoyama
  6. J. Gen. Appl. Microbiol. v.33 Protoplast fusion of Lactobacillus acidophilus and Streptococcus lactis via electric field or chemical induction Reed, W. M.
  7. FEMS Microbiol. Lett. v.44 Transformation of Lactobacillus casei by electroporation Chassy, B. M.;J. L. Fickinger
  8. Streptococcal Genetics Transformation of Streptococcus lactis by electroporation Harlander, S. K.;Ferretti, J. J.(ed.0;Curtiss, R.(ed.)
  9. Food Biotechnol v.5 Bacteriocins, Their role in developing natural products Marugg, I. D.
  10. Trend Food Sci. Tech. v.2 Bacteiocin production by lactic acid bacteria: Potential for use in meat preservation Stiles, M. E.;J. W. Hasting
  11. J. Gen. Microbiol. v.48 Properties of Lactobacillus fermenti bacteriocin De Klerk, H. C.;J. A. Smit
  12. J. Bacteriol. v.167 Characterization and purification of helveticin J and evidence for a chromosomally determined bacteriocin produced by Lactobacillus helveticus 481 Joeger, M.;T. R. Klaenhammer
  13. J. Bacteriol. v.55 Cloning, phenotypic expression and DNA sequence of the gene for lactain F, a bacteriocin produced by Lactobacillus acidophilus Muriana, P. M.;T. R. Klaenhammer
  14. J. Appl. Bacteriol. v.71 Plasmid profile and curing of plasmid in Lactobacillus plantarum strain isolated from green olive fermentations Ruiz-Barba, J. L.;J. C. Piard;R. Jimenez-Diaz
  15. J. Ind. Microbiol. v.4 Bacteiocin plasmids of Pediococcus acidilactici Ray, S. K.;M. C. Johnson;B. Ray
  16. J. Appl. Bacteriol. v.74 Characterization of bacteriocin produced by Pediococcus pentosaceus from wine de Saad, A. M. S.;M. C. M. de Nadra
  17. J. Food Protect. v.42 Role of Lactobacillus in the intestinal tract Sandine, W. E.
  18. Appl. Environ. Microbiol. v.45 Detection and activity of lactacin B, a bacteriocin produced by Lactobacillus acidophilus Barefoot, S. F.;T. R. Klaenhammer
  19. Kor. J. Appl. Microbiol. Biotechnol. v.22 Studies on the Protoplast Formation and Regeneration of Lactobacillus acidophilus 88 Jun, H. K.;K. Heo;Y. B. Jo;H. S. Baik
  20. Appl. Environ. Microbiol. v.57 Bacteriocin-producing strain of Lactococcus lactis subsp. diacitilactis S50 Kojic, M.;J. Svircevic;A. Banina;L. Topisirovic
  21. Phytopathology v.69 Phaseolotoxin transport in E. coli and Salmonella typhimurium via the oligopeptide permease Staskawicz, B. J.;N. J. Panopoulos
  22. Bergey's Manual of Systematic Bacteriology Sneath, P. H. A.;N. S. Mair;M. E. Sharpe;J. G. Holt
  23. Biochemical Tests for Identification of Medical Bacteria(2nd ed.) Jean, F. M.
  24. Agric. Biol. Chem. v.47 Fusion of protoplasts of Streptococcus lactis Okamoto, T.;Y. Fujita;R. Irie
  25. Appl. Environ. Microbiol. v.57 Improved Electroporation and Cloning Vector System for Gram-Positive Bacteria Dunny, G. M.;L. N. Lee;D. J. LeBlanc
  26. Appl. Environ. Microbiol. v.55 Improved Electroporation Efficiency of Intact Lactococcus lactis subsp. lactis Cells Grown in Defined Media Maclntyre, D. A.;S. K. Harlander
  27. Agric. Biol. Chem. v.43 Distribution of β-galactosidase activity among lactic Streptococci Okamoto, T.;T. Morichi
  28. Biochem. Biophis. Acta v.819 Fusion of bacterial spheroplasts by electric fields Ruthe, H. J.;J. Adler
  29. J. Gen. Microbiol. v.126 Protoplast fusion in Streptomyces: Fusions involving ultraviolet-irrediated protoplast Hopwood, D. A.;H. M. Wright