Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effects of Glutamine, Glycine and Nucleosides/Nucleotide Mixture on Intestinal Mucosal Growth in Rats

흰쥐의 소장 점막 세포의 성장에 미치는 Glutamine, Glycine과 Nucleosides/Nucleotide 혼합물의 효과

  • 이선영 (충남대학교 식품영양학과) ;
  • 오현인 (충남대학교 식품영양학과)
  • Published : 1997.02.01
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Abstract

Total parenteral nutritional effect was induced by surgical creation of Thiry-Vella fistula(TVFs) in rats. Glutamine, glycine or nucleosides/nucleotide mixture in solution was injected into the loops for 2, 4, 6, 8 days. Control animals received a 0.9％ saline solution. Results include weight gain, total protein, DNA, ［$^3$H］ thymidine incorporation into DNA, morphometry of the intestine in both TVFs and intestine in continuity. Perfusion of nucleosides/nucleotide mixture into the bypassed loops caused an increase in total protein, DNA content, villous height, villous surface area in loops. The injection of glycine into loops caused an increase in ［$^3$H］ thymidine incorporation but the mean values of the protein and DNA contents were not significantly different from those in group Cont and group Nuc. Overall values for group Gln were slightly higher than those of the control but the differences were not statistically significant. This study suggests that this animal model may be useful for studying the effect of dietary factors on intestinal growth and maturation, separating the direct effect of diet from systemic effect on the intestine.

250g내외의 횐쥐에게 Thiry-Vella fistula를 만든 후 수술과 비경구 영양 효과로 인하여 위축되는 소장의 누공에 각각 nucleosides/nucleotide혼합용액 (Nuc군)이나 glutamine(Gln군), glycine(Gly군)등을 투여하여 소장 점막의 위축 방지나 재생효과가 있는지를 알아보고 자 실험하여 다음과 같은 결과를 얻었다. Thiry-Vella fistula 시술 후 9일 동안 2일에 한 번씩 총 4회 소장 누공으로 주입한 이미노산들과 nucleosides/nucleotide 혼합물의 효과는 누공의 소장 점막에 미치는 직접적인 효과와 비누공 부분에 대한 간접적인 효과로 나누어 볼 수 있다. Nucleosides/nucleotide 혼합물은 소장 누공으로 주입되어 직접 접촉되는 부분의 소장 DNA함량을 유의적으로 증가 시켰으며 단백질 함량도 높은 수치를 보여 주었고 조직 검사 결과 소장 융모의 길이와 표면적을 증가시킨 것으로 나타났다. 그러나 ［$^3$H］ thymidine incorporation의 결과는 대조군과 유의적인 차이를 보이지 않았다. Glycine은 소장 단백질과 DNA에 있어서 대조군보다는 높고 Nuc군보다는 낮은 경향을 보여 주었으나 ［$^3$H］-thymidine incorporation은 누공과 비누공부분에서 모두 가장 높게 나타났으며 특히 비누공부분에서는 유의적으로 증가되어 있었다. Glutamine의 효과는 glycine 투여 효과와 비슷하거나 오히려 떨어지는 것으로 나타났다.

Keywords

References

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