Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Growth Inhibitory Effect and Changes in Membrane Phospholipid Fatty Acid Composition on MG-63 and AZ-521 Human Cancer Cells by Linoleic Acid

리놀레산에 의한 인체암세포의 성장 억제효과 및 암세포막 인지질 지방산 변화

  • 임선영 (부산대학교 식품영양학과 및 암연구소) ;
  • 이숙희 (부산대학교 식품영양학과 및 암연구소) ;
  • 이세윤 (동덕여자대학교 약학연구소) ;
  • 박건영 (부산대학교 식품영양학과 및 암연구소)
  • Published : 1997.08.01
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Abstract

Linoleic acid(LA) was examined to evaluate its potential as a chemotherapeutic agent for MG-63 human osteosarcoma and AZ-521 gastric cancer cells. The treatment of LA(0.005% for 6 days) to the MG-63 and AZ-521 cancer cells inhibited growth of the cancer cells by 54% and 52%, respectively as compared to that of the controls. It also exhibited that LA with 0.01% concentration decreased the [$^3$H] thymidine incorporation by more than 90% in the both cancer cells. In additions we observed morphological changes in MG-63 and AZ-521 cells under inverted microscope, and the changes in membrane fatty acid compositions of the cancer cells when LA was added at the level of 0.005%. The treatment with LA revealed that the contents of 16:0 and 18:0 decreased significantly, but fatty acids that C numbers are more than 20 and unsaturated(20:4, 22:6, and 24:4) increased, concomitantly the morphological changes of the cells were observed.

MG-63 골육암세포 및 AZ-521 인체 위 암세포 성장에 미치는 리놀레산(LA)의 성장 억제효과와 이때 암세포막의 인지질 유리지방산 조성 변화에 대한 연구를 하였다. LA 첨가 농도 0.005%에서 MG-63 암세포와 AZ-521 암세포는 대조군에 비해 각각 54% 및 52%의 암세포 성장이 억제되었다. 또한 LA 첨가(0.01%)시 이들 암세포의 ［$^3$H］ thymindine 결합능은 90% 이상이 억제되어 암세포의 DNA합성이 크게 억제되었다. 한편 LA(0.005 %) 첨가시 각 암세포의 형태학적인 변형을 유발시켰으며 세포막의 인지질 지방산 조성의 변화가 관찰되었다. LA처리는 이런 세포의 형태적 변형과 함께 세포막의 인지질 중 16:0과 18:0의 지방산은 현저히 감소되었으나 탄소수가 20 이상의 탄소수를 가지거나 불포화 도가 높은 지방산(20 : 4, 22 6, 24 : 4)은 증가되었다.

Keywords

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