Journal of Korea Technical Association of The Pulp and Paper Industry (펄프종이기술)

Korea Technical Association of the Pulp and Paper Industry (한국펄프종이공학회)
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Studies on the Recycling Technology of the Waste Paper with Wood Degradable Enzyme(I) - Separation of Crude Enzyme from Wood Degradable fungi -

목질분해효소에 의한 고지의 재활용 기술연구 (제1보) -목질분해균으로부터 조효소의 단리

  • 양재경 (경북대학교 농업과학기술연구소) ;
  • 이중명 (경북대학교 농과대학) ;
  • 엄태진 (경북대학교 농과대학)
  • Published : 1997.03.01
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Abstract

The various culture conditions of Trichoderma viride(ATCC 3454) and Phanerochaete chrysosporium(ATCC 26921) with glucose-pepton medium, Mandels medium, YMG medium for wood degradable enzyme were examined. Mycellium of the two species grew profusely on glucose-pepton medium. Maximum fungal growth was observed about 10days. But CMCase, Fpase, laccase activity in the culture medium with glucose-pepton was not detected. When grown in fermenter culture using Mandels medium, Trichoderma viride produced CMCase and Fpase. Its CMCase activity was 0.15 lU/ml and Fpase activity was 0.3 IU/ml within about 4-6days. Phanerochaete chrysosporium grown in a YMG medium gave the best enzyme activity when they were grown under stationary culture with an atmosphere of 100% oxygen. Levels of laccase activity of 3.0 mull were achieved in stationary culture under 100% oxygen. The enzyme condensation by ultrafiltration method caused a 2-fold(cellulase) and 6-fold(laccase) as compared to control activity.

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