Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Plant Regeneration and Somatic Embryogenesis from Zygotic Embryo-derived Callus of Native Prunus yedoensis in Mt. Halla

한라산 자생 왕벚나무 접합자배 유래의 캘러스로부터 체세포배 형성과 식물체 재분화

  • Published : 1997.11.01
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Abstract

Somatic embryos were induced through embryogenic callus derived from immature zygotic embryo culture of native Prunus yedoensis in Mt. Halla and regenerated into plantlets successfully. Embryogenic callus was induced most effectively on MS medium with 1.0 mg/L 2, 4-D and 0.1 mg/L BAP at an efficiency of approximately 60% using 45 day-old zygotic embryos after full blooming. Globular somatic embryos were induced from embryogenic callus on MS medium with 1.0 mg/L 2, 4-D and 0.1 mg/L BAP and these globular embryos developed to heart-shaped and cotyledonary embryos on hormone-free MS medium. Normal somatic embryos germinated 49% on 1/2 MS medium and the plants regenerated from the somatic embryos were morphologically normal.

한라산에 자생하는 왕벚나무(Prunus yedoensis)의 미성숙 접합자배로부터 배발생 캘러스를 거쳐 체세포배를 유도할 수 있었으며, 이들 체세포배로부터 식물체를 재분화시킬 수 있었다. 배발생 캘러스는 1.0 mg/L 2,4-D와 0.1 mg/L BAP가 혼합 처리된 MS 배지에서 가장 효과적으로 유도되었으며, 그 중에서 90%가 배발생 캘러스였다. 또한 배발생 캘러스는 만개 후 45일된 종자의 접합자배에서 전체 60%가 발생되어 가장 양호하였다. 유도된 배발생 캘러스를 1.0 mg/L 2,4-D와 0.1 mg/L BAP가 혼합처리된 MS 배지에서 4주 간격으로 계대배양하여 구형 또는 심장형으로 발달한 체세포배를 얻을 수 있었고, 이들 체세포배는 식물생장조절제가 첨가되지 않은 MS 배지에서 배양하여 정상적인 자엽을 갖는 체세포배를 얻을 수 있었다. 이들 체세포배들은 배지의 종류에 따라 발아율은 0~49%로 맡은 차이를 보였으나, 식물생장조절제가 첨가되지 않은 1/2 MS 배지에서 49%의 발아율을 보여 가장 양호하게 식물체로 재분화되었다.

Keywords

References

  1. Bull Soc R Bot Belg v.107 La culture de meristemes apicaux de quelques especes de prunus Boxus P;Quoirin M
  2. Plant Physiol v.89 Maturation of soybean somatic embryos and the transition to plantlet growth Bucnneim JA;Colburn SM;Ranch JP
  3. Korean J Plant Tissue Culture v.22 Plant regeneration from immature zygotic embryos of Stewartia koreana Nakai via somatic embryogenesis Choi EG;Park HB;Kim KS;Lee YK
  4. Korean J Plant Tissue Culture v.20 no.5 Structural aspects of somatic embryos derived from cultures zygotic embryos in Acanthopamax senticosus L Choi YE;Soh WY
  5. Korean J Plant Tissue Culture Plant somatic embryogenesis and artificial seeds. Advances in developmental biology and biotechnology of higer plants Guo Z;Gui Y;WY Sho(ed.);JR Liu(ed.);A Komamine(ed.)
  6. Plant Tissue Culture Manual v.A2 The intiation and maintenance of calls cultures of carrot and tobacco Hall GD
  7. Acta Hort v.124 Adventive embryogenesis in pear Janick J
  8. Korean J Plant Tissue Culture v.20 Effects of media, plant growth regulators and dark treatments on in vitro plant regeneration using vegetative bud of Prunus yedoensis Matsumura Kim CS;Koh JG;Cho RM
  9. HortScience v.18 Adventive embryogenesis from leaf explants of apple seedings Liu JR;Sink KC;Dennis FG
  10. J Biol Chem v.193 Protein measurement with the Folin phenol reagent Lowry OH;Rosebrough HJ;Farr AL;Randall RJ
  11. HortScience v.17 In vitro propagation of Nemaguard peach rootstock Miller GA;Coston DC;Demmy EG;Romeo ME
  12. Physiol Plant v.15 A revised medium for rapid growth and bioassays with tobacco tissue culture Murashige T;Skoog F
  13. HortScience v.17 In vitro propagation of ornamental rosaceous plants Norton ME;Boe AA
  14. Korean J Bot v.8 A historical survey on the Prumus yedoensis in Korea Park MK
  15. Korean J Plant Tissue Culture v.19 Enzyme activities in relation to nitrogen assimilation in nicotiana tabacum callus Paek KY;Lee CH
  16. Korean J Plant Tissue Culture v.19 Plant regeneration and somatic embryogenesis from immature embry of Japanese apricot(Prunus mume Sidb et Zucc) Park HB;Choi EG
  17. Planta v.53 Ueber die kontrolle der morphogenese und die induktion von adventive embryonen an gewebekulturen aus karotten Reinert J
  18. HortScience v.17 In vitro propagation of sweet cherry cultivars Snir I
  19. Korean J Plant Tissue Culture v.19 Effect of sorbitol on embryogenic callus and embryoid formation in callus cultures of rice(Oryza sativa L) Sohn JK;Lee GH
  20. Amer J Bot v.45 Growth and organized develpoment of cultured cell. I. Growth and division of freely suspended cells Steward FC;Mapes MO;Smith J
  21. HortScience v.12 Shoot culture for almond and almondpeach hybrid clones in vitro Tabachink L;Kester DE
  22. HortScience v.19 Somatic embryyogenesis and plant regeneration from immature embryos of strawberry Wang D;Wergin WP;Zimmerman RH