Journal of Microbiology and Biotechnology
- Volume 7 Issue 2
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- Pages.157-159
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- 1997
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
A Partial Nucleotide Sequence of Chitin Synthase (CHS) Gene from Rice Blast Fungus, Pyricularia oryzae and Its Cloning
- Hwang, Cher-Won (Department Environmental Microbiology, Handong University) ;
- Park, In-Cheol (Molecular Genetic Division, National Agricultural Science and Technology Institute, RDA) ;
- Yeh, Wan-Hae (Molecular Genetic Division, National Agricultural Science and Technology Institute, RDA) ;
- Takagi, Masamchi (Department of Agricultural Chemistry, The University of Tokyo) ;
- Ryu, Jin-Chang (Molecular Genetic Division, National Agricultural Science and Technology Institute, RDA)
- Published : 1997.04.01
Abstract
A 340-bp chitin synthase gene(CHS) fragment was cloned from the genomic DNA of Pyricularia oryzae using a PCR process with two primer DNAs corresponding to highly conserved sequences within fungal CHS genes. The entire DNA nucleotide sequences of the cloned DNA fragment were determined and analyzed. The amino acid sequences deduced from the nucleotide sequence of the amplified DNA fragment showed 86% homology to that of the Aspergillus fumigatus CHSE gene (9). Using this PCR-amplified DNA, about 2.3 kb of including the PCR fragment of CHSE gene was cloned from genomic library.