The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Cultural Characteristics for Inducing Fruting-body of Isaria japonica

눈꽃동충하초의 자실체 유도를 위한 배양조건

  • Ban, Ki-Won (Department of Applied Biology, Dongguk University) ;
  • Park, Dong-Kyoo (Department of Applied Biology, Dongguk University) ;
  • Shim, Jae-Ouk (Department of Applied Biology, Dongguk University) ;
  • Lee, Youn-Su (Division of Applied Plant Sciences, Kangwon National University) ;
  • Park, Chul-Ho (Division of Applied Plant Sciences, Kangwon National University) ;
  • Lee, Ji-Yul (Institute for Genetic Engineering Konkuk University) ;
  • Lee, Tae-Soo (Department of Biology, University of Inchon) ;
  • Lee, Sang-Sun (Department of Biological Science and Education, Korean National University of Education) ;
  • Lee, Min-Woong (Department of Applied Biology, Dongguk University)
  • 반기원 (동국대학교 응용생물학과) ;
  • 박동규 (동국대학교 응용생물학과) ;
  • 심재욱 (동국대학교 응용생물학과) ;
  • 이윤수 (강원대학교 농업생명과학대 식물응용과학부) ;
  • 박철호 (강원대학교 농업생명과학대 식물응용과학부) ;
  • 이지열 (건국대학교 유전공학연구소) ;
  • 이태수 (인천대학교 생물학과) ;
  • 이상선 (한국교원대학교 생물교육학과) ;
  • 이민웅 (동국대학교 응용생물학과)
  • Published : 1998.09.30
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Abstract

To obtain basic data for mass production of Isaria japonica, cultural characteristics of japonica were investigated by using liquid, solid media and silkworms pupa. Mycelia grew favorably at the temperature of $23{\sim}28^{\circ}C$ on MYG medium with pH 7.0. The fruiting-body of I. japonica was induced below $20^{\circ}C$ in MYG liquid medium (Malt yeast glucose) under fluorescent light. In MYG basal medium mixed with pupal powder of silkworms, the fresh weight of fruiting-bodies was increased with increasing concentration of pupal powder. The highest yield of fruiting bodies was obtained in carbon-rich medium supplemented with pupal powder of silkworm. Also, fruiting-bodies of I. japonica were produced massively on the silkworm pupa placed on the stainless tray in the shortest time. The structure and shape of fruiting-bodies were coral-like, many-branched types with numerous conidiospores.

눈꽃동충하초의 대량 인공재배를 위한 배양적 특징을 조사하기 위하여 기본실험을 수행하였다. 공시 배지로는 액체, 고체의 MYG(Malt yeast glucose) 배지를 이용하였고 균사생장은 $23{\sim}27^{\circ}C$의 범위의 온도에서 비교적 좋은 생장률을 나타내었으며 최적 온도는 $25^{\circ}C$, pH는 7.0에서 가장 좋은 생장을 나타내었다. 자실체 형성을 위한 부산물로서 번데기가루, 한약찌꺼기 등이 적합하였고, 눈꽃동충하초의 대량생산을 위한 간편한 방법으로서 일반 누에번데기를 균현탁액과 gelatin을 혼합한 용액을 접종원으로 이용하여 접종한 후, 배양하였을 때 우수한 자실체 형성을 보여 주었다.

Keywords

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