KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Culture Condition of Entomopathogenic Nematodes Using Galleria mellonella Larva

Galleria mellonella 유충을 이용한 곤충병원성 선충의 배양 조건

  • 김도완 (계명대학교 화학재료공학부) ;
  • 박선호 (강원대학교 공과대학 화학공학과)
  • Published : 1998.02.01
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Abstract

A simple method for the in vivo production of third-stage infective juveniles(IJs) of Steinernema glaseri was developed. Using Galleria mellonella larvae, only IJs can be rapidly generated inadequate quantities for field application. The nematode inoculation concentration and incubation temperature were critically important. The most effective temperature for infectivity of Steinernema glaseri IJs to Galleria mellonella larvae was 33$^\circ C$. However, the total number of menatodes harvested at 25$^\circ C$ about 66,000 IJs per larva was significantly greater than those at other temperatures. The optimal inoculation number of nematodes was 60 to 80 nematodes per host larva. The higher nematode inoculation concentration of 100 IJs per larva caused a rapid decrease in the total number of IJs harvested. As the inoculation medium pH increased, the number of IJs harvested increased and reached about 110,000 IJs per larva at pH 9.0. The pathogenicity of IJs decreased y increasing the salt concentration in the medium.

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References

  1. 한국생물공학회지 v.12 no.3 곤충병원성 선충을 이용한 무공해 생물 농약(총설) 박선호;김도완
  2. Entomophagous Nematodes, in : Biological Plant and Health Protection Poinar,G.O.Jr.
  3. Steinernematid and Heterohabditid Nematodes : A Handbook of Techniques, Southern Cooperative Series Bullentin 331 a Publication of the Nematode Subcommitte of the Southern Regional Project S-135 Entomopathogens for the Use IMP Systems Kaya,H.K.;L.W.Jennifer
  4. J. Nematol. v.25 no.2 Simple In Vivo Production and Storage Methods for Steinernema carpocapsae Infective Juveniles Lindegern,J.E.;K.A.Valero;B.E.Mackey
  5. J. Invertebrate Pathology v.57 Effects of Soil Temperature, Moisture, and Relative Humidity on Entomopathogenic Nematode Persistence Kung,S.P.;R.Gaugler
  6. Indian Journal fo Agricultural Science v.64 no.9 Biocontrol Potential of Native Entomopathogenic Nematodes in Controlling Red Hairy Caterpillar(Amasacta albistriga)(Lepidoptera : Arctiidate) of Groundnut(Arachis hypogaea) Baskaran,R.K.M.;C.V.Sivakumar;M.S.Venugopal
  7. J. Nematol v.25 no.4 Survey of Heterorhabditidate and Steinernmatidae(Rhabditida, Nematoda) in Western Canada Mracek,Z.;J.M.Webster
  8. J. Economic Entomology v.85 no.5 Comparison of Ten Entomopathogenic Nematodes for Control of Sweetpotato Weevil(Coleoptera : Apionidae) Catharine,M.M.;R.K.Jansson
  9. Nematropica v.20 no.1 Use of Entomopathogenous Nematodes of the Families Heterohabditidae and Steinernematidae to Control Banna Moth(Opogona sachari) Pena,J.E.;W.J.Schroeder;L.S.Osborne
  10. J. Economic Entomology v.86 no.3 Laboratory and Field Evaluation of and Entomogenous Nematode(Nematoda : Steinernematidae) for German Cockrach(Dictyoptera : Blattellidae)Control Appel,A.G.;E.P.Benson;J.M.Ellenberger;S.A.Manweiler
  11. 한국토양동물학회지 v.1 no.1 한국산 곤충병원성 선충과 Steinernema 속의 검색표 추호렬;김준범;이동윤
  12. 산림과학논문집 v.51 한국산 곤충기생성 선충의 분포 및 병원성 김준범;박지두;김철수
  13. 산림과학논문집 v.53 남부지방에서 곤충병원성 선충과 곤충병원성 곰팡이의분리 이상명;이동운;추호렬
  14. 한국응용곤충학회지 v.34 no.4 한국산 곤충 병원성 선충(Steinernematidae와 Heterohabditidae)의 지역 농림 해충에 대한 병원성 추호렬;이상명;정부근;박영도;김형환
  15. 석사학위논문, 경상대학교 곤충병원성 선충 Steinernematid 와 Heterorhabditid 및 곤충병원성 곰팡이 Beauveria brongniartii를 이용한 골프장 굼벵이의 생물적 방제 허진
  16. 산림과학논문집 v.53 남부지방 산림 토양에서 분리된 곤충병원성 선충 Steinermema spp. 의 증식과 병원성 이상명;이동운;추호렬
  17. Annu. Rev. Enromol v.39 Entomopathogenic Nematodes Kaya,H.K.;R.Gaugller