KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Revelation of Antitumor Effect in Combination with 5-Fluorocytosine and Extracellular Cytosine Deaminase

5-Fluorocytosine과 세포외 Cytosine Deaminase의 병용투여에 의한 항암효과의 발현

  • Kim, Tae-Hyun (Department of Herbs Foods, Traditional Food Institute, Kyongbuk College of Science) ;
  • Kim, Jung (Department of Microbiology, College of Natural Science, Keimyung University) ;
  • Yu, Tae-Shick (Department of Microbiology, College of Natural Science, Keimyung University)
  • 김대현 (경북과학대학 약용식품학과, 전통발효연구소) ;
  • 김정 (계명대학교 자연과학대학 미생물학과) ;
  • 유대식 (경북과학대학 약용식품학과, 전통발효연구소)
  • Published : 1998.12.01
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Abstract

This study was carried out particularly focusing on he antitumor effect in combination with 5-fluorocytosine(5-FC), antifungal agent, and extracellular cytosine deaminase from Chromobacterium violaceum YK 391 against U-937, K-562 and SNU-C4 cells. While the addition of 10$\mu\textrm{g}$/100 ${\mu}\ell$ of anticancer agent, 5-fluorouracil(5-FU), to U-937, K-562 and SNU-C4 caused the decrease of proliferation 90%, 75% and 93% respectively, the addition of 20 $\mu\textrm{g}$/100 ${\mu}\ell$ of the extracellular cytosine deaminase and 10 $\mu\textrm{g}$/100 ${\mu}\ell$ of antifungal agent 5-FC caused the decrease of proliferation 80%, 70% and 90%, respectively. These results, therefore, reveal that this enzyme has the similar clinical effect for considering of adjuvant antitumor effect. From the above results, the treatment of 5-FC and the cytosine deaminase was very effective and showed the possibility to remove side effects which easily occur by the treatment of 5-FU only. An extracellular cytosine deaminase.

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