Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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A study on determination of novobiocin residues in milk by high performance liquid chromatography

고속액체크로마토그라피를 이용한 우유 중 노보비오신 분석에 관한 연구

  • 김현정 (서울특별시보건환경연구원 축산물부) ;
  • 황래홍 (서울특별시보건환경연구원 축산물부) ;
  • 정지헌 (서울특별시보건환경연구원 축산물부) ;
  • 윤은선 (서울특별시보건환경연구원 축산물부) ;
  • 박노운 (서울특별시보건환경연구원 축산물부) ;
  • 한인규 (서울특별시보건환경연구원 축산물부)
  • Published : 1998.10.01
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Abstract

A method for the determination of noboviocin in milk was presented by high performance liquid chromatography(HPLC). The novobiocin in the spiked sample was extracted with methanol and evaporated under vacuum. After evaporating, the residue was mixed with distilled water for 2$m\ell$, filtrates with 0.45$\mu\textrm{m}$ acrodisc was injected into HPLC. The results obtained were as follows ; 1. The calibration curve of novobiocin was showed constantly linear(r 0.999) in the range of 100~500ng/$m\ell$. 2. The mean recovery rate of novobiocin from the spiked milk sample were 88~98%. 3. The coefficients of variation were 2.6~5.8% 4. The lowest detectable limit of novobiocin was 25ppb.

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