Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지

Callus Induction and Somatic Embryogenesis from Sicyos angulatus L.

야생식물 Sicyos angulatus L.로부터 캘러스 유도 및 체세포배 발생

  • 권순태 (안동대학교 자연과학대학 원예육종학과) ;
  • 조문수 (대구대학교 자연자원대학 원예학과)
  • Published : 1998.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

In order to investigate the possibility of in vitro mass propagation via somatic embryogenesis from Sicyos angulatus L., effects of plant growth regulators and carbon sources on callus induction and somatic embryogenesis were evaluated. Optimal combinations of plant growth regulator for callus induction from cotyledon and inflorescence explants were 2,4-D 2.0 mg/L + BA 0.1 mg/L and 2,4-D 1.0 mg/L + BA 0.1 mg/L in MS basal medium supplemented with sucrose 30 g/L,, respectively. Somatic embryogenesis was observed from cultured inflorescence explants, but it could not be achieved from leaf or cotyledon explants. The most effective plant growth regulators for somatic embryogenesis from callus was NAA 1.0 mg/L + kinetin 10 mg/L in the half strength of MS basal medium supplemented with 20 g/L sucrose.

박과식물과 우수한 접목친화성을 가진 것으로 알려진 야생식물인 Sicyos angulatus L.(일명 '안동오이')의 화기, 자엽 및 잎 조직을 배양하여 캘러스유도 및 체세포배의 획득 가능성을 밝히고 기내배양에 의한 묘의 대량증식에 필요한 기초자료를 얻고자 본 실험을 수행하였다. 자엽이나 화기 절편체로부터 캘러스유도를 위한 가장 적합한 식물생장조절제는 MS 배지에 각각 2,4-D 2.0㎎/L + BA 0.1 ㎎/L와 2,4-D 1.0㎎/L + BA 0.1 ㎎/L를 첨가한 것이었다. 잎 및 자엽 절편체로부터는 체세포배 발생이 되지 않았으나 화기 절편체에서는 체세포배 발생이 관찰되었다. 화기로부터 유래한 캘러스를 NAA 1.0 ㎎/L+ kinetin 1.0 ㎎/L가 첨가된 1/2 MS기본배지에 배양하면 가장 효율적으로 체세포배를 획득할 수 있었고, 탄소원으로는 sucrose 20 g/L가 가장 효율적이었다.

Keywords

References

  1. Korean J Medicinal Crop Sci v.2 Effect of carbon and nitrogen source on somatic embryogenesis in suspension culture of Ligusticum chuanxiang Hort Che YA;Park SU
  2. RDA J Agric Sci v.36 Plant regeneration through embryogenesis of Cnidium officinale Makino Cho HJ;Yang WY;Lee JR;Park YG;Lee SY
  3. Res Rept RAD v.33 no.3 Somatic embryogenesis from inflorescence dericved callus of Oplopanaxelatus NAKAI Cho HJ;Yang WY;Yan DW;Kim YH;Soh WY;Chung TY
  4. Advances in Developmental Biology and Biotechnology of Higher Plants Plant somatic embryogenesis and artifical seeds Guo Z;Gui Y;Soh WY;Lui JR;Komamine A (eds.)
  5. Proc of 24th International Horticultural Congress at Kyoto Studies on the seed germination an ddesease resistance of wild plant, Sicyos angulatus L. as the root stock of cucurbitacea vegetables Hwang JM;Lee SG;Lee WY
  6. Korean J Plant Tissue Culture v.22 Somatic embryogenesis from filament-derived callus of Paeonia Iactiglora PALL Jung JD;Harn JS;Sohn JK
  7. Korean J Plant Tissue Culture v.23 Response of cucumber and Sicyos angulatus L plants and cells to drough and temperature stresses Kwon ST;Cho MS;Oh SM
  8. J Kor Soc Hort Sci. v.30 Somatic embryogenesis and structural aberrancy of embryos in tissue culture of aralia cordata Thumb. Lee KS;Soh WY
  9. Korean J Plant Tissue Culture v.20 Somatic embryogenesis and structural aberrancy of embryos intissue culture of aralia cordata Thumb. Lee KS;Soh WY
  10. J. Kor Soc HortSic v.32 Characteristics of Sicyos andgulatus L. growing wild in Andong area and its potential as root stock for cucurbitaceous crops Lee WH;Kim SB;Kwak BH
  11. Plant Physiol v.137 Differential effects of sucrose, glucose and fructose during somatic embryogenesis in asparagus Levi A;Sink KC
  12. J Exp Bot v.30 NAA-induced organogenesis and embryogenesis in hypocotyl callus of Solanum melongena L. Matsuoka H;Hinata K
  13. Physiol Plant v.15 A revised medium for rapid growth and bioassay with tobacco culture Murashige T;Skoog F
  14. Plant Science v.67 Somatic embryogenesis and plant regeneration from immature inflorescences of several elite inbreds of maize Pareddy DR;Petolino JF
  15. J. Kor Soc HortSci v.30 Effects of several rootstocks on plant growth fruit quality and yield in oriental melon Park JY;Chung HD
  16. Plant Cell Tissue Organ Culture v.5 Somatic embryogenesis and plant regeneration from immature inflorescence of Coxi Iacrymajobi Sun CS;Chu CC
  17. Z Pflanzenphysiol v.97 Embryoid formation and subsequent plantlet regeneration from callus cultures of Thalictrum urbarini Hayata(Rammculaceae) Yang YW;Chang WC
  18. Annals Botany v.65 Somatic embryogenesis and its hormonal regulation in tissue cultures of Freesia refracta Wang L;Huang B;He M;Hao S