KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Quantitative Analysis and Varietal Difference of Cyanidin 3-glucoside in Pigmented Rice

  • Park, Sun-Zik (Department of Agricultural Science, Korea National Open University) ;
  • Lee, Jong-Hoon (Department of Agricultural Science, Korea National Open University) ;
  • Han, Sang-Jun (Department of Agricultural Science, Korea National Open University) ;
  • Kim, Hong-Yeol (Nat'l Crop Experiment Station, Rural Development Administration) ;
  • Ryu, Su-Noh (Nat'l Crop Experiment Station, Rural Development Administration)
  • Published : 1998.09.01
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Abstract

The cyanidin 3-glucoside (C3G) extracted from pigmented rice seeds in 0.5% TFA (Trifluoro acetic acid) -95% ethanol was separated by High Performance Liquid Chromatography (HPLC). A HPLC system using a Develosil ODS-5 column and 0.1 % TFA-$H_2O$～0.1 % TFA-$CH_3CN$ gradient elution was selected for separation and quantitative determination of C3G. Regression equation obtained for the standard content of C3G pigment was as Y=21.95293$^*$X-14.726771 (r=0.99$^{**}$). Using this method, 326 domestic and introduced collections were evaluated for the C3G content. The Korean bred cultivar 'Heugjinjubyeo', showed highest C3G content (552 mg/100g seed) among the tested cultivars. Among the pigmented rice cultivars ten cultivars were selected for containing a high content of C3G. The content of C3G per 100g seeds was in high order as follows: Heugjinjubyeo (552mg)>Cheng Chang (321mg)>Kilimgeugmi (240mg)>PI160979-2 (224mg)>Hong Shei Lo (221mg)>Heugnambyeo (191 mg)>Mitak =PIl60979-1 (186mg)>Suwon425 (163mg)>Sanghaehyanghyeolla (108mg). The C3G pigment was not detected in the common white rice cultivars.

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