Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Elucidation of Anti-tumor Initiator and Promoter Derived from Seaweed-2: Investigation of Seaweed Extracts Suppressing Mutagenic Activity of PhIP and MeIQx

해조류 중의 anti-tumor initiator 및 promoter의 해석-2: PhIP와 MeIQx의 돌연변이원성을 억제하는 해조 추출물 소재의 연구

  • PARK Young-Beom (Department of Food Science and Technology, Pukyong National University) ;
  • KIM In-Soo (Department of Food Science and Institute of Marine Industry, Gyeongsang National University) ;
  • YOO Sung-Jae (Department of Food Science and Technology, Pukyong National University) ;
  • AHN Jong-Khan (Department of Food Science and Technology, Pukyong National University) ;
  • LEE Tae-Gee (Department of Food Science and Technology, Pukyong National University) ;
  • PARK Douck-Chon (Department of Food Science and Technology, Pukyong National University) ;
  • KIM Seon-Bong (Department of Food Science and Technology, Pukyong National University)
  • 박영범 (부경대학교 식품공학과) ;
  • 김인수 (경상대학교 식품과학과 해양산업연구소) ;
  • 유승재 (부경대학교 식품공학과) ;
  • 안종관 (부경대학교 식품공학과) ;
  • 이태기 (부경대학교 식품공학과) ;
  • 박덕천 (부경대학교 식품공학과) ;
  • 김선봉 (부경대학교 식품공학과)
  • Published : 1998.07.01
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Abstract

The present study was conducted to prepare seaweed extracts suppressing mutagenic activity of 2-amino-1-methyl-6-phenylimidazo[4,5-b] pyridine ( PhIP ) ana 2-amino-3,8-dimethylmidazo[4,5-f]quinoxaline (MeIQx) derived from cooked meat produce. The tumor initiation activity of PhIP and MeIQx was assayed with Ames method using Salmonella typhimurium TA98 in the presence of S-9 mixtures before and after addition of methanol-solubles of seaweed, such as, Phaeophyta; Undaria pinnatifida, Ecklonia stolonifera Ecklonia cava, Laminaia japonica Sargassum fulvellum, Sargassum hornezi, Sargassum miyabei, Sargassum thunbergii, Agarum cribrosum and Hizikia fusifomis, Rhodophpei Porphyra yezoensis, Grateloupia eiliptiut Lomentraria catenata, Ploemium telfairiae and Glarilaria verrucosa, Chlorophyta; Codium fragile, Enteromorpha compresa and Ulva pertusa. Among seaweed tested, Phaeophyta was shown the higher desmutagenic activity than Rhodophyta and Chlorophyta. E. stolonifera, E. cava and S. miyabei, among Phaeophyta exerted the stronger desmutagenic activity (above $90\%$/2 mg). The ethyl acetate, diethyl ether and chlomform extracts except water extracts from E. stolonifera exhibited a high desmutagenic activity. The ethyl acetate extract of E. stolonifera which showed highest activity was fractionated with Sephadex LH20 column chromatography to give active fraction A-7, which showed desmutagenicity of $90\%$/mg against PhIP and $80\%$/mg against MeIQx. The active fraction had the absorbance at 207.7 and 232nm.

해조류 추출물을 이용하여 가열 식품 유래의 돌연변이 유발물질인 PhIP와 MeIQx에 대한 돌연변이원성 억제소재를 개발하기 위하여 연구한 결과, PhIP와 MeIQx에 대한 돌연변이 억제작용은 갈조류가 녹조류 및 홍조류에 비해 우수하였다 특히 갈조류 중에서는 감태, 곰피, 다시마 및 미야베모자반에서 그 효과가 우수하였다. 그 중에서 곰피를 diethyl ether, chloroform, ethyl acetate 및 수용성 획분으로 분획하였을 때, 수용성 획분을 제외한 비극성 획분에서 억제효과가 강하게 나타났는데, ethyl acetate 획분에서 강하였다. 곰피의 ethyl acetate 획분을 Sephadex LH20으로 분획 하여 분리 한 결과, 활성 획 분 A-7은 PhIP에 대하여는 $90\%$/mg 이상, MeIQx에 대하여는 $80\%$/mg의 억제효과를 나타내었고, 207.7 및 232nm에서 흡수파장을 나타내었다.

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