Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Application of FITC-conjugated lectin probes for the recognition and differentiation of some Korean coastal red tide microalgae

  • Cho Eun Seob (Harmful Algae Biology Division, National Fisheries Research and Development Institute) ;
  • Seo Gwi Moon (Department of Biotechnology, Pukyong National University) ;
  • Lee Sam Geun (Harmful Algae Biology Division, National Fisheries Research and Development Institute) ;
  • Kim Hak Gyoon (Harmful Algae Biology Division, National Fisheries Research and Development Institute) ;
  • Lee Sang Jun (Biotechnology Division, National Fisheries Research and Development Institute) ;
  • Rhodes Lesley L. (Cawthron Institute) ;
  • Hong Yong-Ki (Department of Biotechnology, Pukyong National University)
  • Published : 1998.12.01
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Abstract

Harmful micro algae isolated from Korean coastal waters, were tested with FITC-conjugated lectins and observed by epifluorescent microscopy to distinguish each other. Strain-specific sugar composition at the cell surface was suggested by the affinity of lectins to different microalgae. The micro algae Cochlodinium polykrikoides (CP-1) and Gymnodinium $A_3\;(GA_{3-1}\;1)$, are morphologically similar, but exhibited different binding activity with the lectins ECA, HPA and WGA. In Peridiniales, the micro alga Alexandrium tamarense (AT) bound HPA and WGA, but Scrippsiella trochoidea (ST-1) did not bind those lectins. Three species of Prorocentrum also exhibited different binding specificity with HPA, PHA and SBA. A non­toxic Korean isolate of Heterosigma akashiwo (HA-2) bound ConA, PEA and UEA. These results suggest that lectins are useful in discriminating morphologically similar species, as well as different species or strains within the same genus.

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