The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지

Isolation Method of Mushroom Infesting Pests from Mushroom-Growing Compost

버섯배지에서 버섯해충류 분리방법

  • Lee, Heung-Su (Div. of Plant Environment, Kyongnam Agricultural Research & Extension Services) ;
  • Kim, Kyu-Jin (Dept. of Agri. Biol., Chonnam National University) ;
  • Song, Geun-Woo (Div. of Plant Environment, Kyongnam Agricultural Research & Extension Services) ;
  • Kim, Jin-Ho (Dept. of Pl. Resources and Environ., Gyeongsang National University)
  • Published : 1999.08.30
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Abstract

We investigated the isolation method of mushroom infesting pests, Lycoriella mali, Coboldia fuscipes, Histiostoma sp. from mushroom-growing compost. Sugar solution of different densities (0, 10, 20, 30, 40, 50%) was tested to provide a means of seperating mushroom pests from the compost media. Thus, 40% sugar solution was suitable for isolation. The sieve size to entrap the pests was $30{\sim}140$ mesh; Lycoriella mali was trappped mainly $30{\sim}65$ mesh sieve, Coboldia fuscipes was caught mainly $30{\sim}100$ mesh sieve, Histiostoma sp. was trapped mainly $65{\sim}140$ mesh sieve. An isolation procedure was as follows; The infested compost was disintegrated in water and poured onto a set of 16, 30, 80, 140-mesh sieve. The material caught in the sieve is added in 40% sugar solution and then most compost particle were massed at the bottom while the supernatant contains mushroom pests. The upperlayer material was poured into a Seperatory funnel and the sediment at the bottom is drained off. The remaining material are washed off examination dish for study.

느타리버섯이 자라는 배지에서 파리류인 Lycoriella mali, Coboldia fuscipes와 응애류인 Histiostoma sp 분리하는 방법을 조사하였다. 배지에서 버섯해충을 분리하기 위해 설탕용액 0, 10, 20, 30, 40, 50%에서 각 해충의 구배를 알아본 결과 설탕 40% 용액이 배지와 버섯해충을 분리하기에 가장 적합하였다. 배지에 들어있는 해충을 걸러내기 위한 체의 크기를 조사한 결과 Lycoriella mali는 $30{\sim}65$ mesh체에서 Coboldia fuscipes는 $30{\sim}100$ mesh체에서 Histiostoma sp.는 $65{\sim}140$ mesh체에서 많이 조사되었다. 이상의 결과로 배지에서 버섯해충을 추출하는 방법을 정리하면 오염된 배지를 물에 불려 부스러뜨리고 16, 30, 80, 140 mesh체에 걸러낸다. 체에 걸러진 입자를 설탕용액 40%가 담겨진 용기에 옮겨 부으면 대부분의 배지입자는 아래로 가라앉고 용액의 상층부로 일부 입자와 버섯파리류 유충, 번데기와 응액류가 떠오르게 된다. 상층부의 것을 Seperatory funnel로 옮겨 붓고 아래로 가라앉은 침전물을 마개를 조절하면서 흘러 내려준다. 남아 있는 버섯해충을 옮겨 붓고 현미경 하에서 조사한다.

Keywords

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