Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Inhibition of Nitric Oxide Production by Coumarins from Peucedanum japonicum in LPS-Activated RAW 264.7 cells

갯기름나물의 쿠마린에 의한 RAW 264.7 세포주의 Nitric Oxide 생성 저해활성

  • Choi, Hee-Cheol (Cellular Response Modifier R.U., Korea Research Institute of Bioscience and Biotechnology) ;
  • Rho, Tae-Cheol (Cellular Response Modifier R.U., Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Bo-Yeon (Cellular Response Modifier R.U., Korea Research Institute of Bioscience and Biotechnology) ;
  • Ko, Hack-Ryong (Cellular Response Modifier R.U., Korea Research Institute of Bioscience and Biotechnology) ;
  • Oh, Won-Keun (Cellular Response Modifier R.U., Korea Research Institute of Bioscience and Biotechnology) ;
  • Seong, Chang-Keun (Department of Food Science and Technology, Chungnam National University) ;
  • Mheen, Tae-Ick (Cellular Response Modifier R.U., Korea Research Institute of Bioscience and Biotechnology) ;
  • Ahn, Jong-Seog (Cellular Response Modifier R.U., Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Hyun-Sun (Cellular Response Modifier R.U., Korea Research Institute of Bioscience and Biotechnology)
  • 최희철 (생명공학연구소 세포반응조절 R.U.) ;
  • 노태철 (생명공학연구소 세포반응조절 R.U.) ;
  • 김보연 (생명공학연구소 세포반응조절 R.U.) ;
  • 고학룡 (생명공학연구소 세포반응조절 R.U.) ;
  • 오원근 (생명공학연구소 세포반응조절 R.U.) ;
  • 성창근 (충남대학교 식품공학과) ;
  • 민태익 (생명공학연구소 세포반응조절 R.U.) ;
  • 안종석 (생명공학연구소 세포반응조절 R.U.) ;
  • 이현선 (생명공학연구소 세포반응조절 R.U.)
  • Published : 1999.06.30
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Abstract

During the screening for inhibitors of nitric oxide production in LPS-activated macrophage, RAW 264.7 cells. Five coumarins were isolated from chloroform extract of the root of Peucedanum japonicum. They were identified as praeruptorin A (1), xanthotoxin (2), psoralen (3), isopimpinellin (4), bergapten (5) on the basis of spectroscopic methods. The $IC_{50}$ values for nitrite production by activated macrophages were about $1.5\;{\mu}g/ml$ (1), $0.3\;{\mu}g/ml$ (2), $1.0\;{\mu}g/ml$ (3), $25\;{\mu}g/ml$ (4), $25\;{\mu}g/ml$ (5), respectively. However, the inducible nitric oxide synthase (iNOS) was not inhibited by treatment with these compounds. Their inhibitory effect on nitric oxide production was resulted from the supperssion of iNOS expression.

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