Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Isolation of Grb2-Shc Interaction Inhibitory Compounds from the Leaves of Eucommia ulmoides O.

두충(Eucommia ulmoides O.)잎으로부터 Grb2-Shc 결합저해 활성물질의 분리

  • Hahn, Jae-Taek (Kyung Hee University, Department of Life Sciences and Institute of Life Sciences) ;
  • Ahn, Eun-Mi (Kyung Hee University, Department of Life Sciences and Institute of Life Sciences) ;
  • Bang, Myun-Ho (Kyung Hee University, Department of Life Sciences and Institute of Life Sciences) ;
  • Nam, Ji-Youn (Korea Research Institute of Bioscience and Biotechnology, KIST) ;
  • Kwon, Byoung-Mog (Korea Research Institute of Bioscience and Biotechnology, KIST) ;
  • Baek, Nam-In (Kyung Hee University, Department of Life Sciences and Institute of Life Sciences)
  • 한재택 (경희대학교 생명과학부 및 생명과학연구원) ;
  • 안은미 (경희대학교 생명과학부 및 생명과학연구원) ;
  • 방면호 (경희대학교 생명과학부 및 생명과학연구원) ;
  • 남지연 (한국과학기술연구원 생명공학연구소) ;
  • 권병목 (한국과학기술연구원 생명공학연구소) ;
  • 백남인 (경희대학교 생명과학부 및 생명과학연구원)
  • Published : 1999.06.30
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Abstract

The MeOH extracts obtained from the leaves of Eucommia ulmoides were solvent-fractionated with EtOAc, n-BuOH, and $H_20$, successively. From the EtOAc extract showing Grab2-Shc inhibitory activity, a flavonol and a lignan compounds were isolated through repeated silica gel column chromatographies. By interpretation of several spectral data and adaptation of acetylation method, the chemical structures of the compounds were determined as 5,7,3',4'-tetrahydroxy-flavonol (quercetin), and 4,4',8',9-tetrahydroxy-3,3'-dimethoxy-7,9'-cyclolignan {(-)-olivil)}, respectively. The compounds exhibited $IC_{50}$ values in Grb2-Shc inhibitory activity to be 93 and $210\;{\mu}mole/l$, respectively.

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