Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Selection of RAPD Marker for Growth of Seedlings at Low Temperature in Rice

Kim, Kyung-Min;Park, Gyu-Hwan;Kim, Jin-Ho;Kwon, Yong-Sham;Sohn, Jae-Keun

  • Published : 1999.06.30

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Abstract

We have developed a polymerase chain reaction (PCR)-based assay that could effectively reduce the time period required to screen and select the cold tolerance gene of rice seedlings under field conditions. The two specific random amplified polymorphic DNA (RAPD) fragments for the assay were identified on the basis of quantitative trait loci (QTL) analysis which were found to be tightly linked to cold sensitivity. The two RAPD fragments, $OPT8_{600}$ in the cold sensitivity rice cultivar 'Dular (indica)' and $OPU20_{1200}$ in the resistance rice cultivar 'Toyohatamochi (japonica)', were identified after screening 11 RAPD fragments using 2 random primers on the genomic DNAs of 'Dular' and 'Toyohatamochi'. These primers, when used in a multiplexed PCR, specifically amplified a 0.6 kb and a 1.2 kb fragment in the sensitive and resistant rice cultivars, respectively. When this assay was performed on the genomic DNAs of 16 japonica, 3 Tongil (indica/japonica), and 2 indica rice cultivars, the primers amplified a 0.6 kb fragment in all of the cold sensitivity rice cultivars or 1.2 kb fragment in all of the resistance ones. These markers can be of potential use in the marker-assisted selection (MAS) for cold tolerance in rice seedling. As screening for resistance can now be conducted independent of the availability of low temperature, the breeding of cold tolerance cultivars can be hastened.

Keywords

References

  1. Diversity v.6 Molecular markers: Important tools for plant genetic resource characterization Andersen, W.R.;Fairbanks, D.J.
  2. Oryza sativa L. Bull. Inst. Trop. Agr. Kyushu Univ. v.5 Studies on the chlorosis under low temperature condition in rice Choung, P.V.;Omura, T.
  3. Mol. Cells v.6 Random amplified polymorphic DNA and restriction fragment length polymorphism analysis to differentiate races of the rice blast fungus, Pyricularia oryzae Hong, S.M.;Kang, K.Y.;Kim, N.S.;Kang, S.W.;Kim, K.K.
  4. Theor. Appl. Genet. v.95 More on the efficiency of marker-as sited selection Hospital, F.;Moreau, L.;Lacoudre, F.;Charcosset, A.;Gallais, A.
  5. SABRAO Journal v.17 Expression of chlorosis at low temperature in rice plants Kaimori, N.;Takahashi, N.
  6. Korean J. Breed. v.29 Construction of linkage map using RAPD and RFLP markers in rice Kim, K.M.;Sohn, J.K.
  7. Korean J. Breed. v.29 Analysis of a QTL associated with cold tolerance at seedling stage of rice by RAPD markers Kim, K.M.;Sohn, J.K.;Kato, A.;Oono, K.
  8. Report of a Rice Cold Tolerance Workshop, IRRI Screening methods for cold tolerance at crop experiment station phytotron at Chuncheon Lee, J.H.
  9. Jpn. J. Breed. v.41 Genetic control of tolerance to chilling injury at seedling stage in rice, Oryza sativa L. Nagamine, T.
  10. Jpn. J. Breed. v.40 Genetic variation of chilling injury at seedling stage in rice, Oryza sativa L. Nagamine, T.;Nakagahra, M.
  11. Theor. Appl. Genet. v.91 DNA markers tightly linked to a gall midge resistance gene (Gm2) are potentially useful for marker-aided selection in rice breeding Nair, S.;Bentur, J.S.;Prasada Rao, U.;Mohan, M.
  12. Report of a Rice Cold Tolerance Workshop, IRRI Breeding strategy for coldtolerant rices Nanda, G.S.;Seshu, D.V.
  13. Extraction of total cellular DNA from plants, algae and fungi;Plant Molecular Biology Manual, D1 Rogers, S.O.;Bendich, A.J.;Gelvin, S.B.(ed.);Schilperoort, R.A.(ed.);Verma, D.P.S.(ed.)
  14. Bull. Natl. Ins. Agri. Sci. v.D34 Physiological study of growth inhibition in rice plant as affected by low temperature. II. Physiological mechanism and varietal difference of chilling injury in rice plant Tajima, K.;Amemiya, A.;Kabaki, N.
  15. Restriction fragment length polymorphic DNA markers;Methods in Enzymology, 218 Williams, M.N.V.;Pande, N.;Nair, S.;Mohan, M.;Bennett, J.;Wu, R.(ed.)
  16. Plant Physiol. v.110 Cooltemperature-induced chlorosis in rice plants Yoshida, R.;Kanno, A.;Sato, T.;Kameya, T.
  17. Laboratory Manual for Physiological Studies of Rice Yoshida, S.;Forno, D.A.;Cock, J.H.;Gomez, K.A.