Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

Relationship of Wheat Polyphenol Oxidase Activity to Color of Asian Raw Noodles

Lee, Won-Jong;Kim, Sung-Hoon;Shelton, David R.;Kim, Mi-Jung

  • Published : 19991200

⟨ Previous Next ⟩

Abstract

Polyphenol oxidase (PPO) has been implicated in darkening reactions that limit the acceptability of wheat-based end products, such as Asian noodles. A simple, rapid procedure was developed for determining activity of PPO in wheats without for seed grinding. This method may assist wheat breeders in making decisions about advancing experimental lines. Seeds (0.4 g) were crushed with a plier, and a freshly prepared L-DOPA solution was added. The mixture was incubated for 5 min at $37^{\circ}$ and filtered through a syringe filter (25 mm cellulose acetate, pore size 0.2 micrometer). Absorbance of the reaction solution was measured at 475 nm. The method correlated well (r=0.86, p<0.000l) with PPO assay performed on ground grain suspensions. Twenty five samples consisting of four hard red winter wheat and one hard white wheat were harvested in 1998 at five locations in Wyoming. PPO activity in wheat samples was affected by both variety and growing location. The activity was significantly influenced by the interaction of variety and location effects. PPO activity correlated well with the rate of decrease in brightness ($L^*$) of 25 raw Asian noodle sheets at 4 hr of storage (r=0.75, p<0.000l) and increase in yellowness ($b^*$) after 24 hr of storage at $21^{\circ}C$ (r=0.69, p<0.0001).

Keywords

References

  1. Phytochemistry v.18 Directed oxidases and related enzymes Mayer, A.M.;Harel, E.
  2. Direct oxidase and related enzymes;The Biochemistry of Plants, Vol. 2 Butt, V.S.;Stumpf, P.K.(ed.);Conn, E.E.(ed.)
  3. SABRAO Newl. v.3 Phenol color reaction as an indicator of chapatti quality in wheat Abrol, Y.P.;Uprety, D.C.;Ram, A .;Tikoo, S.
  4. Flat breads;Wheat: Chemistry and Technology, Vol. 2 Faridi, H.;Pomeranz, Y.(ed.)
  5. Can. Inst. Food Sci. Technol. v.14 Development of a high extraction flow for the GRL Pilot Mill to evaluate Canadian wheat potential for the Chinese market Dexter, J.E.;Preston, K.R.;Matsuo, R.R.;Tipples, K.H.
  6. Can. J. Plant Sci. v.72 A comparison of methods for the prediction of Cantonese noodle color Kruger, J.E.;Morgan, B.;Matsuo, R.R.;Preston, K.R.
  7. Cereal Chem. v.72 Discoloration of dough for oriental noodles Baik, B.K,;Czuchajoska, Z.;Pomeranz, Y.
  8. Cereal Chem. v.58 Polyphenol oxidase activities of hard red winter, soft red winter, hard red spring, white common, club, and durum wheat cultivars Lamkin, W.M.;Miller, B.S.;Nelson, S.W.;Taylor, D.D.;Lee, M.S.
  9. Cereal Chem. v.74 Variation in polyphenol oxidase and quality characteristics among hard white wheat and hard winter wheat samples Park, W.J.;Shelton, D.R.;Peterson, C.J.;Martin, T.J.;Kachman, S.D.;Wehling, R.L.
  10. J. Cereal Sci. v.4 Measurement of polyphenol oxidase activity in wheat-milling fractions Marsh, D.R.;Galliard, T.
  11. Cereal Chem. v.70 Distribution of polyphenol oxidase in flout millstreams of Canadian common wheat classes milled to three extraction rates Hatcher, D.W.;Kruger, J.E.
  12. Cereal Chem. v.71 A whole seed assay for polyphenol oxidase in Canadian prairie spring wheats and its usefulness as a measure of noodle darkening Kruger, J.E.;Hatcher, D.W.;DePauw, R.
  13. Cereal Chem. v.76 Measurement of the time-dependent appearance of discolored spots in alkaline noodles by image analysis Hatcher, D.W.;Symons, S.J.;Kruger, J.E.
  14. Approved Methods of the AACC AACC
  15. Abstract No. 67 presented at the 83rd Annual Meeting of American asociation of Cereal Chemists Distribution of PPO activity among a large number of hexaploid wheat genotypes using an improved L-DOPA assay Morris, C.F.;Anderson, J.V.;Bettge, A.D.;Corell, M.E.
  16. SAS Users Guide SAS lnstitude, Inc.
  17. Cereal Chem. v.53 Changes in the polyphenol oxidase of wheat during kernel growth and maturation Kruger, J.E.
  18. J. Cereal Sci. v.19 Comparison of polyphenol oxidase activities in wheats and flour from Australian and U.S. cultivars Baik, B.K.;Czuchajowska, Z.;Pomeranz, Y.