YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Characterization of Polysaccharides from Artemisia capillaris and Artemisia sylvatica

사철쑥과 그늘쑥의 다당류 연구

  • 황은주 (성균관대학교 약학대학 천연물약품학 연구실) ;
  • 권학철 (성균관대학교 약학대학 천연물약품학 연구실) ;
  • 정칠만 (성균관대학교 약학대학 천연물약품학 연구실) ;
  • 문형인 (성균관대학교 약학대학 천연물약품학 연구실) ;
  • 김선여 (농촌진흥청 농업과학원 잠사곤충부) ;
  • 지옥표 (성균관대학교 약학대학 천연물약품학 연구실) ;
  • 이강노 (성균관대학교 약학대학 천연물약품학 연구실)
  • Published : 1999.08.01
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Abstract

Two polysaccharides, ACP-UMP and ACP-ULF, were purified from the aerial part of Artemisia capillaris by anion-exchange chromatography, ultrafiltration, and gel filtration chromatography. The polysaccharides appeared to be homogenious from the results of HPLC. The molecular weights of ACP-UMF and ACP-ULF were estimated to be 16305.92 D and 3292.26 D, respectively, by MALDI-TOF MS. The sugar compositions were determined by GC to be arabinose 10.05%, xylose 1.67%, mannose 5.45G, galactose 39.06%, glucose 15.43% for ACP-UMF and arabinose 11.60%, xylose 11.15%, mannose 6.37% galactose 32.47%, glucose 18.35% for ACP-ULF. A polysaccharide, SP-M was determined to be 2462.52 D by MALDI-TOF MS. SP-M consisted mainly of rhamnose 36.49%, arabinose 29.00%, and glucose 19.38%. Incubation of CCl4-intoxicated hepatocytes with ACP-UMF reduced the levels of glutamic pyruvic transaminase (GPT) and cellular malondialdehyde (MDA) to 62.8% and 23.8%. ACP-ULF also reduced the levels of GPT and MDA to 46.1% and 38.1% and 26.3%, respectively.

Keywords

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