Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
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Cracking Hen's Egg for Transgenesis, without Cracking Them

정자에 의한 외래 DNA의 계란내 도입: 유전자 변환 닭 생산을 위한 장애 극복

  • 이기석 (고려대학교 생명과학부, 생명공학원) ;
  • 김기동 (고려대학교 생명과학부, 생명공학원) ;
  • 이상호 (고려대학교 생명과학부, 생명공학원)
  • Published : 1999.06.01
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Abstract

Hen's eggs have been regarded as one of the best animal bioreactors to produce biologically active peptides originated from many organisms including human. Despite the last decade's efforts to produce transgenic chicken for any commercial purposes, the results so far reported are very disappointing, indicating that hen's eggs are very difficult to crack for transgenesis. Comparatively large female gamete with enormous amount of yolk may be one of the major obstacles in achieving a similar feat to those of other vertebrate species including mouse, sheep, fish and frog. The delay or less efficiency evidenced may instruct to try an alternative way of gens transfer into chicken egg. Sperm-mediated gene transfer is one of them, and may require a great deal of understanding of mechanisms involved in early fertilization and embryonic development. In other animals where the technique was successful, basic mechanisms have been well studied and established only by painstaking efforts for decades. This paper discusses the accumulated knowledge on early fertilization mechanism in the chicken and how can this information be utilitzed to find the alternative gene transfer in making transgenic chicken.

Keywords

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