Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Surface-activity and Environmental Characteristics of Biosurfactant Produced by Pseudomonas aeruginosa JRT-4

Pseudomonas aeruginosa JRT-4에 의해 생산된 Biosurfactant의 계면활성 및 환경적 특성

  • 황경아 (LG화학 생활과학연구소) ;
  • 이정래 (LG화학 생활과학연구소) ;
  • 김상종 (서울대학교 미생물학과) ;
  • 김윤석 (LG화학 생활과학연구소) ;
  • 안호정 (LG화학 생활과학연구소)
  • Published : 1999.04.01
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Abstract

Pseudomonas aeruginosa JRT-4 strain was used as a biosurfactant-producing microorganism in this study. It was one of the microorganisms isolated from the sewage sludge, the main and branch streams of Han river. The surface tension of the culture broth of P. aeruginosa JRT-4 decreased to 30mN/m. The crude biosurfactant was obtained from the culture broth by acid precipitation, solvent extraction, evaporation, and freeze drying. The CMC value of the crude biosurfactant was 0.006%(w/v). From analysis of the chemical structure of biosurfactant, it was determined as rhamnolipid 1 and 3 structures by FAB mass spectrometer. In the washing test for artificially contaminated textiles, the biosurfactant showed better bleachness than the two chemically synthesized surfactant, LAS and SLES. Finally, the biodegradation and ecotoxidolorical tests showed that the biosurfactant was readily biodegradable in the environment and a mild material for microorganisms and green algae.

Keywords

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