Bile Salt Deconjugation Activity of Lactobacillus Strains Isolated from Yogurt Products

요구르트에서 분리한 Lactobacillus들의 담즙산염 분해 능력

  • 김근배 (한국야쿠르트 중앙연구소) ;
  • 이재환 (한국야쿠르트 중앙연구소) ;
  • 임광세 (한국야쿠르트 중앙연구소) ;
  • 허철성 (한국야쿠르트 중앙연구소) ;
  • 배형석 (한국야쿠르트 중앙연구소) ;
  • 백영진 (한국야쿠르트 중앙연구소) ;
  • 김현욱 (서울대학교 동물자원과학과)
  • Published : 1999.10.01

Abstract

To investigate bile salt hydrolase activities of the bacterial strains isolated from fermented milk products, 21 strains of Lactobacillus were tested for their abilities to produced cholic acid from taurocholic and glycocholic acids. The production of cholic acid was measured by HPLC analysis during the growth in broth media for 24hrs. All strains of Lactobacillus acidophilus and L. plantarum deconjugated both taurocholate and glycocholate, whereas none strains of L. delbrueckii subsp. bulgaricus, L. casei subsp. casei, L. casei subsp. rhamnosus, L, reuteri did. L. acidophilus stains isolated from yogurts had the higher decojugation activities on glycocholate than taurocholate, however, L. acidophilus 1009 isolated from the human intestine showed the similar deconjugation activities on both taurocholate and glycocholate.

Keywords

References

  1. Biochim. Biophys. Acta v.202 Degradation of steroid by intestinal bacteria. I. Deconjugation of bile salts Aries, V.;M. J. Hill
  2. J. Dairy Sci. v.79 Hypocholesterolemic action of Lactobacillus acidophilus ATCC 3121 and calcium in swine with hypercholesterolemia induced by diet De Rodas, B. Z.;S. E. Gilliland;C. V. Williams
  3. J. Appl. Bacteriol. v.79 Significance of bile salt hydrolytic activities of lactobacilli De Smet, I.;L. Van Hoorde;M. Vande Woestyne;H. Christiaens;W. Verstraete
  4. Br. J. Nutr. v.79 Cholesterol lowering in pigs through enhanced bacterial bile salt hydrolase activity De Smet, I.;P. De Boever;W. Verstraete
  5. Int. J. Food Microbiol. v.40 Characterization and selection of probiotic lactobacilli for a preliminary minipigs feeding trial and their effect on serum cholesterol levels, faeces pH and faeces moisture content Du Troit, M.;C. M. A. P. Franz;L. M. T. Dicks;U. Schillinger;P. Haberer;B. Warlies;F. Ahrens;W. H. Holzapfel
  6. Appl. Environ. Microbiol. v.33 Deconjugation of bile acids by intestinal lactobacilli Gilliand, S. E.;M. L. Speck
  7. J. Lipid Res v.29 Purification and characterization of bile salt hydrolase from Clostridium perfringens Gopal-Srivastava, R.;P. B. Hylemon
  8. Appl. Environ. Microbiol. v.61 Purification and characterization of conjugated bile salt hydrolase from Bifidobacterium longum BB536 Grill, J. P.;F. Schneider;J. Crociani;J. Ballogue
  9. J. Pediatr. Gastroenterol. Nutr. v.20 Intestinal microbial bile acid transformation in healthy infants Jonsson, G.;A. C. Midtvedt;A. Norman;T. Midtvedt
  10. J. Biochem. v.106 Purification and characterization of a new hydrolase for conjugated bile acids, chenodeoxycholyltaurine hydrolase from Bacteroides vulgatus Kawamoto, K.;I. Horibe;K. Uchida
  11. J. Biochem. v.84 A new hydrolase specific for taurine-conjugates of bile acids Kobashi, K.;I. Nishizawa;T. Yamada;J. Hase
  12. J. Bacteriol. v.172 Characterization and purification of bile salt hydrolase from Lactobacillus sp. strain 100-100 Lundeen, S.;D. C. Savage
  13. FEMS Microbiol. Lett. v.94 Characterization of an extracellular factor that stimulates bile salt hydrolase activity in Lactobacillus sp. strain 100-100. Lundeen, S.;D. C. Savage
  14. Microbiol. Immunol. v.25 Deconjugation of bile salts by Bacteroides and Clostridium Masuda, N.
  15. Am. J. Clin. Nutr. v.27 Microbial bile acid transformation Midtvedt, T.
  16. J. Intestinal. Microbiol. v.11 Bile acid and disease Oosua, T.
  17. Clin. Chim. Acta v.119 A simple reverse phase high pressure liquid chromatographic determination of conjugated bile acids acids in serum and bile using a novel radial compression separation system Ruben, A. T.;G. P. VanBerge-Henegouwen
  18. SAS User's Guide, Statistics (6th ed.) SAS Institute, Inc.
  19. Biochim. Biophys. Acta v.452 Purification and characterization of bile salt hydrolase from Bacteroides fragilis ssp. fragilis Stellwag, E. J.;P. B. Hylemon
  20. J. Dairy Sci. v.77 Absence of cholic acid 7α-dehydroxylase activity in the strains of Lactobacillus and Bifidobacterium Takahashi, T.;M. Morotomi
  21. J. Dairy Sci. v.76 Relationship among bile tolerance, bile salt deconjugation and assimilation of cholesterol by Lactobacillus acidophilus Walker, D. K.;S. E. Gilliland
  22. Chromatogr. v.25 A quantitative extraction method for conjugated bile acids from human serum using reversed-phase octadecyl bonded silica cartridges for HPLC analysis Zhang, Y. Y.;W. Swododnik;P. Furst;H. Ditschuneit
  23. Appl. Environ. Bacteriol. v.56 Modeling of the bacterial growth curve Zwietering, M. H.;I. Jongeburger;F. M. Rombouts;K. van't Riet