Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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A Whole Cell Bioluminescent Biosensor for the Detection of Membrane-Damaging Toxicity

  • Park, Sue-Hyung (Department of Environmental Science and Engineering, Kwangju Institute of Science and Technology(K-JIST)) ;
  • Gu, Man-Bock (Department of Environmental Science and Engineering, Kwangju Institute of Science and Technology(K-JIST))
  • Published : 1999.03.01
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Abstract

The recombinant bacteria strain DPD2540, containing a fabA::luxCDABE fusion, was used to detect the toxicity of various chemicals in this study. Membrane damaging agents such as phenol, ethanol, and cerulenin induced a rapid bioluminescent response from this strain. Other toxic agents, such as DNA-damaging or oxidative-damaging chemicals, showed a delayed bioluminescent response in which the maximum peak appeared over 150 min after induction. This strain was also tested for measurement of toxicity in field samples such as wastewater and river water effluents.

Keywords

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