KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Isolation and Characterization of Phosphorus Accumulating Acinetobacter CW3

인 축적균 Acinetobacter CW3의 분리 및 특성

  • 심성훈 (영남대학교 화학공학 및 공업화학부) ;
  • 류원률 (영남대학교 화학공학 및 공업화학부) ;
  • 이영호 (영남대학교 화학공학 및 공업화학부) ;
  • 김정목 (대경대학 환경공업과) ;
  • 조무환 (영남대학교 화학공학 및 공업화학부)
  • Published : 1999.02.01
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Abstract

A highly effective phosphorus accumulating bacterium named Acinetobacter CW3 was isolated from the nature by using Winogradsky columns. The optimal cultivation conditions of Acinetobacter CW3 in shaking flask were determined as $20^{\circ}C$, pH 7, 200rpm, 18.5mg $PO_4$-P/L. Acientobacter CW3 could remove phosphorus completely in 12hours for a batch culture at optimal cultivation condition. This bacterium could uptake phosphorus on aerobic condition and release it on anaerobic condition.

인 축적능이 우수한 균주를 자연계로부터 위노그라드스키 칼럼을 이용해 분리하여 Acinetobacter 속으로 동정하였고, Aci-netobacter CW3로 망명하였다. 인 축적균주의 배양초기조건은 $20^{\circ}C$. pH 7, 200rpm, 18.5mg $FO_4-P/\ell$가 최적임을 보였다. Acinetobacter CW3은 회분배양에서는 최적조건에서 12시간만에 인의 제거가 완료되었다. 이 균주는 호기조건에서는 인을 균주 내부에 과량 축적하고 첨가조건에서 인을 다시 방출함을 보였다.

Keywords

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