Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antimutagenic and Anticancer Effects of Ramaria botrytis(Fr.) Rick Extracts

싸리버섯 추출물의 항돌연변이성 및 암세포 성장 억제 효과

  • 이갑랑 (영남대학교 식품영양학과) ;
  • 김현정 (영남대학교 식품영양학과) ;
  • 이인선 (계명대학교 식품가공학과)
  • Published : 1999.12.01
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Abstract

The inhibitory effect of Ramaria botrytis on the mutagenicity in Salmonella assay and cytotoxicity on cancer cell were studied. Ramaria botrytis methanol extracts showed antimutagenic effects of 60~90% on B(a)P and AFB1 in S. typhimurium TA98 and TA100. These extracts showed 73~85% antimutagenicity on TA100 against MNNG. The methanol extracts with strong antimutagenic activities were further fractionated by ethylacetate and water, the ethylacetate fraction were found to be stronger antimutagen icity against MNNG than water fraction. Ramaria botrytis methanol extracts and ethylacetate fraction revealed the highest cytoxicity against HT 29 human colon adenocarcinoma cells in which cell growth was inhibited by 57~88% and 69~94% at 0.25~1.0mg/ml, respectively. These methanol extract and ethyl acetate fraction exhibited 53~79% and 66~87% inhibition against HepG2 human hepatocarcinoma cells, respectively. But water fraction showed only 10~24% inhibition. However, these extract and fractions did not show cytotoxic effect against human chang liver cells. From these results, it is considered that Ramaria botrytis has stronger antimutagenic and anticancer effects in vitro.

Keywords

References

  1. In Genetic toxicology of the diet Dietary desmutagens kada,T.;Inoue,T.;Morita,K.;Namiki,M.;Knudsen,I.(ed.)
  2. In Nutrition and cancer prevention General introduction: Rational for the nutritional prevention of cancer Micozzi,M.S.;Tangrea,J.A.
  3. Korean mushrooms Kim,S.S.;Kim,Y.S.
  4. J. Korean Soc. Food Nutr. v.3 Study on the tasty constituents and minerals in Clavariaceae botrytis Seoh,J.H.;Cho,S.Y.;Lee,S.W.
  5. Korean J. Nutr. v.5 A study on the amino acid contents of edible mushrooms Pyo,M.Y.;Ro,I.H.
  6. Korean J. Mycol. v.10 Studies on constituents of higer fungi of Korea(ⅩⅩⅩⅣ) Antitumor components of Ramaria formosa Yoo,I.S.;Woo,M.S.;Choi,E.C.;Kim,B.K.
  7. Mutat. Res. v.113 Revised methods for the Salmonella mutagenicity test Ames,B.N.;Maron,D.M.
  8. J. Immunological Methods v.70 Rapid colorimetric assay for cel viability : Application to the quantitation of cytotoxic and growth inhibitory lymphokines Green,L.M.;Reade,J.L.;Ware,C.F.
  9. J. Korean Soc. Food Sci. Nutr. v.28 Antimutagenic effect of the fruting body and the mycelia extracts of Coprinus comatus Kim,H.J.;Lee,B.H.;Kim,O.M.;Bae,J.T.;Park,S.H.;Park,D.C.;Lee,K.P.
  10. J. Korean Sco. Food Sci. Nutr. v.21 Inhibitory effect of green-yellow vegetables on the mutagenicity in Salmonella assay system and on the growth of AZ-521 human gastric cancer cells Park,Y.K.;Lee,K.I.;Rhee,S.H.
  11. Korean J. Food Sci. Technol. v.30 Effect of Platycodon grandiflorum DC extract on the growth of cancer cell lines Lee,J.Y.;Hwang,W.I.;Lim,S.T.